Any covalent dimer would appear at roughly twice the parent mass, and on a cysteine-containing sequence at twice the parent minus two hydrogens. The envelope on this record shows no series at either offset.
dimer_disulfide
Intermolecular disulfide dimers, their retention and their reducibility.
Section 1
Contribution
Comments technically on records submitted by others. Annotations are signed and are not moderated for agreement.
Records submitted
0
n=0
Annotations
65
n=65
Median purity submitted
suppressed
n=0
n<3 — see individual records
Suppliers covered
0
n=0
This handle has submitted no records. Annotators comment on other people's submissions and are not required to submit.
Section 2
Annotations
Signed technical comments on records. The index does not moderate annotations for agreement with its own arithmetic, and several of these disagree with it. Where a handle has made the same point on several records, one instance is shown and the rest are reachable from the records themselves.
An intermolecular disulfide dimer elutes late and reduces cleanly. If the named table shows one, a non-reducing versus reducing comparison converts an unexplained impurity into an explained one.
The index caps the verification status where an essential measurement for the compound class was not made. Pairing is not measurable by the panel on this record and the status reflects it.
An intermolecular dimer appears at roughly twice the parent mass and reduces cleanly. It is a concentration and oxidation-condition artefact from the folding step rather than a sequence problem.
Reduction of the sample before injection collapses dimer into monomer and is a two-line experiment. Comparing the reduced and non-reduced chromatograms answers most of these questions.
Showing 5 distinct comments of 65 annotations by this handle.