AX-2025-019340 · Dihexa · HYB lot HYB-2501-T6290

Section 1

Results

Measured against the index acceptance specification for the neuroactive and cognitive peptides class. A specification is a decision the index made; a result is a measurement. See where each threshold came from.

Parameter	Method	Result	Unit	Index spec
Purity	RP-HPLC, 15 min gradient, 214 nm	99.71	% area	≥ 98.5	PASS
Total impurities	RP-HPLC, sum of reported peaks	0.29	% area	≤ 1.5	PASS
Largest single impurity	RP-HPLC — des-hexanoyl (−98 Da)	0.11	% area	≤ 1.00	PASS
Identity — theoretical monoisotopic mass	calculated from molecular formula	504.3312	Da	—	NOT TESTED
Identity — observed monoisotopic mass	LC-MS, unit resolution (single quadrupole)	504.3293	Da	—	NOT TESTED
Mass accuracy	observed vs theoretical	-3.7	ppm	± 15	PASS
Total peptide content	by difference from the gravimetric mass balance	92.59	%w/w	—	NOT TESTED
Target-compound content	total peptide content × purity	92.32	%w/w	—	NOT TESTED
Gross fill (as weighed)	five-place balance, under nitrogen	19.87	mg	20 mg declared	NOT TESTED
Recovered target peptide	gross fill × target content	18.344	mg	20 mg claim	NOT TESTED
Recovery against label claim	recovered ÷ claim	91.7	%	90.0 – 110.0	PASS
Water content	Karl Fischer, coulometric, duplicate	7.11	%	≤ 12.0	PASS
Counter-ion	no ionisable counter-ion expected	N/A	—	not applicable	NOT TESTED
Residual solvent + inorganic residue	estimated — see /methods/residual-solvents-q3c/	0.30	%	—	NOT TESTED
Endotoxin	not ordered on this submission	not determined	—	≤ 5.0	NOT TESTED
Sterility	not ordered on this submission	not determined	—	no growth	NOT TESTED
Appearance	visual, diffuse light, black and white background	White lyophilised cake, intact	—	white to off-white intact cake	PASS
Reconstitution clarity	reconstituted per submitter procedure	Visible particulates, did not clear	—	clear, no visible particulates	FAIL
pH (reconstituted, 1 mg/mL)	potentiometric, two-point calibration	7.23	—	2.5 – 8.0	PASS
Retention time (observed)	RP-HPLC, 15 min gradient	6.60	min	6.61 ± 0.30 (normalised)	PASS
Peak asymmetry (10 % height)	Ph. Eur. 2.2.46	1.20	—	0.80 – 1.50	PASS
Plate count, main peak	N = 5.54 (t/w½)²	67,354	—	≥ 20,000	PASS
Resolution to nearest impurity	vs [D-Ile]-epimer	0.67	—	no index criterion	REPORTED

Section 2

Chromatogram and peak integration

The figure is drawn from the table. It is not an independent illustration — if a number in the table changed, the trace would change with it.

Figure 1. RP-HPLC trace, 15 min gradient, 214 nm. Drawn from the peak-integration table below: peak positions are the reported retention times, peak areas are the reported area percentages, peak widths follow the reported plate count of 67,354, and skew follows the reported asymmetry factor of 1.20. Baseline noise is seeded from the report ID and is illustrative. The lower panel is the same trace at a stated vertical expansion with the slow gradient drift subtracted, because at full scale a 0.11 % impurity against a 99.71 % main peak is a line two pixels tall; the main peak runs off-scale there by design.

#	Peak	RT	Area (µV·s)	% area	Δ mass (Da)
1	Dihexa — main peak	6.60	4,846,215	99.71	—
2	des-hexanoyl (−98 Da)	5.39	5,346	0.11	-98.074
3	free acid (+1 Da)	6.73	5,346	0.11	+0.984
4	[D-Ile]-epimer	6.53	1,944	0.04	0.000
5	hexanoyl bis-adduct (+98 Da)	7.75	1,458	0.03	+98.074
Closure check — purity + Σ impurities				100.00	CLOSES

Reporting threshold 0.05 % area. Peaks below it are not reported and are therefore excluded from the total, which overstates purity by their sum — see peak integration.

Section 3

Mass spectrum and identity

Theoretical monoisotopic mass 504.3312 Da, computed from C27H44N4O5. All ppm figures on this site are against monoisotopic values — see why that matters.

Figure 2. ESI charge-state envelope, unit-resolution instrument. Sticks are placed at m/z = (M + z × 1.007276)/z for the observed neutral mass of 504.3293 Da. Sodium (+21.982/z) and potassium (+37.956/z) adducts are shown where the parent charge state exceeds 25 % relative abundance; they are adducts of the same molecule and are not impurities.

Charge	Theoretical m/z	Observed m/z	Δ m/z	Rel. abundance (%)
1+	505.3385	505.3366	-0.0019	100.0

Section 4

Gravimetric mass balance

Water, counter-ion, residual and total peptide content are exhaustive and mutually exclusive categories. They must account for 100 % of the cake mass. The index closes this to within 0.02 pp and the build asserts it.

Component	Determination	%w/w of cake
Water	Karl Fischer	7.11
Counter-ion	none expected	0.00
Residual solvent + inorganic residue	estimated	0.30
Total peptide content	by difference	92.59
Total		100.00 CLOSES

Supplier certificate, as declared

The supplier's own figures for this lot, beside the independent measurement. Divergence is assessed against the batch median, not against this single report — see rule AX-R05.

Figure	Supplier COA	This report	Index note
Purity (% area)	99.62	99.71	divergence -0.02 pp vs batch median
Total impurities (% area)	0.38	0.29
Largest single impurity (% area)	0.14	0.11
Water (%)	5.5	7.11
Counter-ion (%)	6.6	N/A
Peptide content (%w/w)	87.3	92.59
Endotoxin (EU/mg)	not stated	not determined
Method stated on certificate	YES	—	column, gradient and wavelength given

Section 5

Provenance and caveats

Where a sample came from changes the weight of the evidence. The index treats it as a first-class field.

Sample provenance: Buyer-sourced
Evidentiary weight: Strongest available evidence in this index.
Chain of custody: Domestic warehouse despatch → submitter (2–8 °C, 28 d) → laboratory (courier, cold pack, 7 d) → assay.
Assay date: 21 Apr 2025
Submitted to the index: 12 May 2025
Laboratory: Kestrel Bioanalytical, Melbourne, Australia
Method basis: 15 min gradient · unit-resolution MS
Verification status: IDENTITY CONSISTENT

What this report does not establish

That the compound is not a near-isobaric alternative. Identity was confirmed at unit mass resolution, which cannot exclude species differing by 1 or 2 Da. The specific alternatives not excluded are named in the identity block above.
That the material is free of bacterial endotoxin. Endotoxin was not measured. No chromatographic result bears on it.
That the material is sterile. Sterility was not tested.
That any other vial in the same lot has the same composition. One vial was tested. Within-lot vial-to-vial variation in this index has a median of 0.28 pp and a 90th percentile of 0.74 pp.
That any other lot from the same supplier has the same composition. Batch-to-batch variation is larger than within-batch variation for every supplier here.
That the material is suitable for administration to a human or an animal. These are research compounds. No result on this site is a safety assessment and the index takes no position on use.
That the material was manufactured by the supplier that sold it. Contract synthesis is normal in this market and twenty-nine batches in the index fingerprint to a bulk from a different company than the one named on the label.

Section 6

Annotations 2

Signed technical comments from index contributors. Not a discussion forum — annotations question or contextualise the result, and the data team replies where a reply is warranted.

isotope_cluster12 May 2025

Nothing here rules out a positional isomer with the same formula. Mass cannot; only chromatography can, and the gradient length on this record is the relevant fact for that.

schema_v212 May 2025

Both the theoretical and the observed monoisotopic mass are in the published dataset, so the ppm figure can be re-derived rather than trusted. That is the intent of publishing both.

Section 7

Related records

Same batch — lot HYB-2501-T6290 n=6

Report	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2025-019341	HYB-2501-T6290	Janoshik	2025-Q2	99.48	7.26	93.6	Buyer-sourced	0	PASS
AX-2025-019342	HYB-2501-T6290	VendorInvestigate	2025-Q2	99.57	7.16	—	Buyer-sourced	1	MARGINAL
AX-2025-019343	HYB-2501-T6290	VendorInvestigate	2025-Q2	99.39	6.83	96.2	Buyer-sourced	0	PASS
AX-2025-019344	HYB-2501-T6290	Kestrel	2025-Q2	99.93	6.63	—	Buyer-sourced	0	PASS
AX-2025-019345	HYB-2501-T6290	Kestrel	2025-Q2	99.93	6.79	—	Buyer-sourced	0	PASS

Batch rollup and reproducibility assessment →

Cross-sections

Other recent Dihexa records in the index.
Report	Supplier	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-041370	WWB	WWB-2601-C4091	Kestrel	2026-Q2	99.93	11.05	—	Buyer-sourced	0	PASS
AX-2026-041521	QSC	QSC-2512-D5887	Janoshik	2026-Q2	99.64	6.16	—	Laboratory-retained	0	PASS
AX-2025-018407	WWB	WWB-2507-N1598	Janoshik	2025-Q3	99.61	9.31	—	Buyer-sourced	0	PASS
AX-2025-019342	HYB	HYB-2501-T6290	VendorInvestigate	2025-Q2	99.57	7.16	—	Buyer-sourced	1	MARGINAL
AX-2025-018408	WWB	WWB-2507-N1598	Janoshik	2025-Q3	99.79	8.96	—	Buyer-sourced	0	PASS
AX-2025-019344	HYB	HYB-2501-T6290	Kestrel	2025-Q2	99.93	6.63	—	Buyer-sourced	0	PASS
AX-2025-019343	HYB	HYB-2501-T6290	VendorInvestigate	2025-Q2	99.39	6.83	96.2	Buyer-sourced	0	PASS
AX-2025-019345	HYB	HYB-2501-T6290	Kestrel	2025-Q2	99.93	6.79	—	Buyer-sourced	0	PASS

Other recent HYB records in the index.
Report	Compound	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-042083	MOTS-c	HYB-2606-D7858	Medutest	2026-Q3	99.93	2.02	—	Buyer-sourced	0	PASS
AX-2026-042090	MOTS-c	HYB-2606-D7858	Janoshik	2026-Q3	99.57	2.36	—	Buyer-sourced	1	FAIL
AX-2026-042084	MOTS-c	HYB-2606-D7858	Janoshik	2026-Q3	99.28	2.79	107.3	Buyer-sourced	0	PASS
AX-2026-042087	MOTS-c	HYB-2606-D7858	Janoshik	2026-Q3	99.54	3.05	—	Buyer-sourced	0	PASS
AX-2026-042085	MOTS-c	HYB-2606-D7858	Janoshik	2026-Q3	99.51	2.31	—	Buyer-sourced	0	PASS
AX-2026-042088	MOTS-c	HYB-2606-D7858	PeptideMeter	2026-Q3	99.22	2.21	—	Buyer-sourced	0	PASS
AX-2026-042086	MOTS-c	HYB-2606-D7858	Janoshik	2026-Q3	99.93	3.02	—	Buyer-sourced	1	MARGINAL
AX-2026-042091	CJC-1295 with DAC	HYB-2608-E7391	Janoshik	2026-Q3	99.38	5.53	—	Buyer-sourced	0	PASS