AX-2025-020148 · MOTS-c · ERP lot ERP-2508-M9453

Section 1

Results

Measured against the index acceptance specification for the metabolic and mitochondrial peptides class. A specification is a decision the index made; a result is a measurement. See where each threshold came from.

Parameter	Method	Result	Unit	Index spec
Purity	RP-HPLC, 25 min gradient, 214 nm	99.25	% area	≥ 98.0	PASS
Total impurities	RP-HPLC, sum of reported peaks	0.75	% area	≤ 2.0	PASS
Largest single impurity	RP-HPLC — Met1 sulfoxide (+16 Da)	0.32	% area	≤ 1.00	PASS
Identity — theoretical monoisotopic mass	calculated from molecular formula	2173.0918	Da	—	NOT TESTED
Identity — observed monoisotopic mass	LC-MS, unit resolution (single quadrupole)	2173.1311	Da	—	NOT TESTED
Mass accuracy	observed vs theoretical	+18.1	ppm	± 15	FAIL
Total peptide content	by difference from the gravimetric mass balance	91.97	%w/w	—	NOT TESTED
Target-compound content	total peptide content × purity	91.28	%w/w	—	NOT TESTED
Gross fill (as weighed)	five-place balance, under nitrogen	10.73	mg	10 mg declared	NOT TESTED
Recovered target peptide	gross fill × target content	9.794	mg	10 mg claim	NOT TESTED
Recovery against label claim	recovered ÷ claim	97.9	%	90.0 – 110.0	PASS
Water content	Karl Fischer, coulometric, duplicate	2.07	%	≤ 9.0	PASS
Counter-ion (acetate)	anion-exchange ion chromatography	5.61	%	≤ 12.0	PASS
Residual solvent + inorganic residue	estimated — see /methods/residual-solvents-q3c/	0.35	%	—	NOT TESTED
Endotoxin	not ordered on this submission	not determined	—	≤ 5.0	NOT TESTED
Sterility	not ordered on this submission	not determined	—	no growth	NOT TESTED
Appearance	visual, diffuse light, black and white background	White lyophilised cake, intact	—	white to off-white intact cake	PASS
Reconstitution clarity	reconstituted per submitter procedure	Clear after 90 s gentle swirling, no particulates	—	clear, no visible particulates	PASS
pH (reconstituted, 1 mg/mL)	potentiometric, two-point calibration	6.13	—	2.5 – 8.0	PASS
Retention time (observed)	RP-HPLC, 25 min gradient	9.00	min	8.82 ± 0.30 (normalised)	PASS
Peak asymmetry (10 % height)	Ph. Eur. 2.2.46	0.89	—	0.80 – 1.50	PASS
Plate count, main peak	N = 5.54 (t/w½)²	73,307	—	≥ 20,000	PASS
Resolution to nearest impurity	vs des-Met1 (−131 Da)	1.35	—	no index criterion	REPORTED

Section 2

Chromatogram and peak integration

The figure is drawn from the table. It is not an independent illustration — if a number in the table changed, the trace would change with it.

Figure 1. RP-HPLC trace, 25 min gradient, 214 nm. Drawn from the peak-integration table below: peak positions are the reported retention times, peak areas are the reported area percentages, peak widths follow the reported plate count of 73,307, and skew follows the reported asymmetry factor of 0.89. Baseline noise is seeded from the report ID and is illustrative. The lower panel is the same trace at a stated vertical expansion with the slow gradient drift subtracted, because at full scale a 0.32 % impurity against a 99.25 % main peak is a line two pixels tall; the main peak runs off-scale there by design.

#	Peak	RT	Area (µV·s)	% area	Δ mass (Da)
1	MOTS-c — main peak	9.00	4,823,858	99.25	—
2	Met1 sulfoxide (+16 Da)	8.68	15,553	0.32	+15.995
3	bis-sulfoxide (+32 Da)	8.45	12,637	0.26	+31.990
4	des-Met1 (−131 Da)	9.18	4,860	0.10	-131.040
5	deamidated (+1 Da)	8.75	3,402	0.07	+0.984
Closure check — purity + Σ impurities				100.00	CLOSES

Reporting threshold 0.05 % area. Peaks below it are not reported and are therefore excluded from the total, which overstates purity by their sum — see peak integration.

Section 3

Mass spectrum and identity

Theoretical monoisotopic mass 2173.0918 Da, computed from C101H152N28O22S2. All ppm figures on this site are against monoisotopic values — see why that matters.

Figure 2. ESI charge-state envelope, unit-resolution instrument. Sticks are placed at m/z = (M + z × 1.007276)/z for the observed neutral mass of 2173.1311 Da. Sodium (+21.982/z) and potassium (+37.956/z) adducts are shown where the parent charge state exceeds 25 % relative abundance; they are adducts of the same molecule and are not impurities.

Charge	Theoretical m/z	Observed m/z	Δ m/z	Rel. abundance (%)
4+	544.2802	544.2901	0.0099	8.0
3+	725.3712	725.3843	0.0131	52.8
2+	1087.5532	1087.5728	0.0196	100.0
1+	2174.0991	2174.1384	0.0393	57.1

Section 4

Gravimetric mass balance

Water, counter-ion, residual and total peptide content are exhaustive and mutually exclusive categories. They must account for 100 % of the cake mass. The index closes this to within 0.02 pp and the build asserts it.

Component	Determination	%w/w of cake
Water	Karl Fischer	2.07
Counter-ion (acetate)	ion chromatography	5.61
Residual solvent + inorganic residue	estimated	0.35
Total peptide content	by difference	91.97
Total		100.00 CLOSES

Supplier certificate, as declared

The supplier's own figures for this lot, beside the independent measurement. Divergence is assessed against the batch median, not against this single report — see rule AX-R05.

Figure	Supplier COA	This report	Index note
Purity (% area)	98.72	99.25	divergence -0.29 pp vs batch median
Total impurities (% area)	1.28	0.75
Largest single impurity (% area)	0.80	0.32
Water (%)	0.4	2.07
Counter-ion (%)	not stated	5.61
Peptide content (%w/w)	94.8	91.97
Endotoxin (EU/mg)	0.27	not determined
Method stated on certificate	NO	—	no method section — the result cannot be reconciled against any other

Section 5

Provenance and caveats

Where a sample came from changes the weight of the evidence. The index treats it as a first-class field.

Sample provenance: Buyer-sourced
Evidentiary weight: Strongest available evidence in this index.
Chain of custody: Retail purchase → submitter (ambient, 3 d) → laboratory (courier, cold pack, 4 d) → assay.
Assay date: 13 Aug 2025
Submitted to the index: 26 Aug 2025
Laboratory: Medutest, Ljubljana, Slovenia
Method basis: 25 min gradient · unit-resolution MS
Verification status: IDENTITY CONSISTENT

What this report does not establish

That the compound is not a near-isobaric alternative. Identity was confirmed at unit mass resolution, which cannot exclude species differing by 1 or 2 Da. The specific alternatives not excluded are named in the identity block above.
That the material is free of bacterial endotoxin. Endotoxin was not measured. No chromatographic result bears on it.
That the material is sterile. Sterility was not tested.
That any other vial in the same lot has the same composition. One vial was tested. Within-lot vial-to-vial variation in this index has a median of 0.28 pp and a 90th percentile of 0.74 pp.
That any other lot from the same supplier has the same composition. Batch-to-batch variation is larger than within-batch variation for every supplier here.
That the material is suitable for administration to a human or an animal. These are research compounds. No result on this site is a safety assessment and the index takes no position on use.
That the material was manufactured by the supplier that sold it. Contract synthesis is normal in this market and twenty-nine batches in the index fingerprint to a bulk from a different company than the one named on the label.

Section 6

Annotations 4

Signed technical comments from index contributors. Not a discussion forum — annotations question or contextualise the result, and the data team replies where a reply is warranted.

ms_confirm26 Aug 2025

The index records this as identity not established. That is a statement about the evidence, not about the material, and the distinction is the whole reason the status vocabulary has four values instead of two.

esi_tof26 Aug 2025

The purity, water and counter-ion figures on this record stand regardless of the mass question. Only the identity limb is affected, and the status field says exactly that.

buyer_sourced26 Aug 2025

No relationship between submitter and supplier is declared on this record, and the contributor register holds none. That is the default and it is what the provenance class asserts.

coa_template26 Aug 2025

The document that arrived with this order is on file and its fields are scored on the supplier's documentation rubric. That score describes what a buyer receives, which is the useful version.

Section 7

Related records

Same batch — lot ERP-2508-M9453 n=12

Report	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2025-020137	ERP-2508-M9453	Janoshik	2025-Q3	98.88	1.80	—	Buyer-sourced	0	PASS
AX-2025-020138	ERP-2508-M9453	Janoshik	2025-Q3	98.51	2.14	95.6	Buyer-sourced	0	PASS
AX-2025-020139	ERP-2508-M9453	Kestrel	2025-Q3	99.07	2.67	—	Buyer-sourced	0	PASS
AX-2025-020140	ERP-2508-M9453	Janoshik	2025-Q3	98.84	2.47	—	Buyer-sourced	0	PASS
AX-2025-020141	ERP-2508-M9453	Janoshik	2025-Q3	99.02	2.13	—	Buyer-sourced	0	PASS
AX-2025-020142	ERP-2508-M9453	Janoshik	2025-Q3	99.00	2.34	—	Supplier-supplied	1	FAIL
AX-2025-020143	ERP-2508-M9453	Nordanalyt	2025-Q3	99.22	2.36	95.0	Buyer-sourced	0	PASS
AX-2025-020144	ERP-2508-M9453	Janoshik	2025-Q3	99.68	2.87	—	Supplier-supplied	0	PASS
AX-2025-020145	ERP-2508-M9453	Janoshik	2025-Q3	98.72	1.66	—	Buyer-sourced	0	PASS
AX-2025-020146	ERP-2508-M9453	Nordanalyt	2025-Q3	99.04	2.69	99.7	Buyer-sourced	0	PASS
AX-2025-020147	ERP-2508-M9453	Nordanalyt	2025-Q3	98.78	2.14	—	Buyer-sourced	0	PASS

Batch rollup and reproducibility assessment →

Cross-sections

Other recent MOTS-c records in the index.
Report	Supplier	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-042083	HYB	HYB-2606-D7858	Medutest	2026-Q3	99.93	2.02	—	Buyer-sourced	0	PASS
AX-2026-042090	HYB	HYB-2606-D7858	Janoshik	2026-Q3	99.57	2.36	—	Buyer-sourced	1	FAIL
AX-2026-042084	HYB	HYB-2606-D7858	Janoshik	2026-Q3	99.28	2.79	107.3	Buyer-sourced	0	PASS
AX-2026-042087	HYB	HYB-2606-D7858	Janoshik	2026-Q3	99.54	3.05	—	Buyer-sourced	0	PASS
AX-2026-042085	HYB	HYB-2606-D7858	Janoshik	2026-Q3	99.51	2.31	—	Buyer-sourced	0	PASS
AX-2026-042088	HYB	HYB-2606-D7858	PeptideMeter	2026-Q3	99.22	2.21	—	Buyer-sourced	0	PASS
AX-2026-042086	HYB	HYB-2606-D7858	Janoshik	2026-Q3	99.93	3.02	—	Buyer-sourced	1	MARGINAL
AX-2026-042081	HYB	HYB-2606-D7858	Nordanalyt	2026-Q3	99.68	3.31	96.5	Laboratory-retained	0	PASS

Other recent ERP records in the index.
Report	Compound	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-042706	Survodutide	ERP-2605-E9865	PeptideMeter	2026-Q3	98.38	5.33	103.5	Buyer-sourced	0	PASS
AX-2026-042704	Tesamorelin	ERP-2604-W9463	Janoshik	2026-Q3	99.02	4.08	—	Buyer-sourced	1	MARGINAL
AX-2026-042705	Survodutide	ERP-2605-E9865	Janoshik	2026-Q3	98.24	5.36	92.3	Buyer-sourced	0	PASS
AX-2026-042708	GHK-Cu	ERP-2601-F9816	Nordanalyt	2026-Q2	98.88	3.87	91.5	Buyer-sourced	1	MARGINAL
AX-2026-042694	Liraglutide	ERP-2601-P8985	Kestrel	2026-Q2	99.25	4.35	107.3	Buyer-sourced	1	MARGINAL
AX-2026-042707	GHK-Cu	ERP-2601-F9816	Janoshik	2026-Q2	98.53	4.70	96.3	Buyer-sourced	1	MARGINAL
AX-2026-042711	GHK-Cu	ERP-2601-F9816	Kestrel	2026-Q2	98.53	3.84	89.7	Laboratory-retained	2	MARGINAL
AX-2026-042722	Tirzepatide	ERP-2512-T9616	Medutest	2026-Q2	99.18	2.07	—	Buyer-sourced	1	MARGINAL