AX-2026-041318 · ARA-290 · WWB lot WWB-2511-S3009

Section 1

Results

Measured against the index acceptance specification for the tissue-repair and immunomodulatory peptides class. A specification is a decision the index made; a result is a measurement. See where each threshold came from.

Parameter	Method	Result	Unit	Index spec
Purity	RP-HPLC, 25 min gradient, 214 nm	99.93	% area	≥ 98.0	PASS
Total impurities	RP-HPLC, sum of reported peaks	0.07	% area	≤ 2.0	PASS
Largest single impurity	RP-HPLC — des-Gln truncate (−128 Da)	0.04	% area	≤ 1.00	PASS
Identity — theoretical monoisotopic mass	calculated from molecular formula	1189.6096	Da	—	NOT TESTED
Identity — observed monoisotopic mass	LC-MS, unit resolution (single quadrupole)	1189.6313	Da	—	NOT TESTED
Mass accuracy	observed vs theoretical	+18.2	ppm	± 15	FAIL
Total peptide content	by difference from the gravimetric mass balance	90.08	%w/w	—	NOT TESTED
Target-compound content	total peptide content × purity	90.02	%w/w	—	NOT TESTED
Gross fill (as weighed)	five-place balance, under nitrogen	3.99	mg	4 mg declared	NOT TESTED
Recovered target peptide	gross fill × target content	3.592	mg	4 mg claim	NOT TESTED
Recovery against label claim	recovered ÷ claim	89.8	%	90.0 – 110.0	FAIL
Water content	Karl Fischer, coulometric, duplicate	4.90	%	≤ 10.0	PASS
Counter-ion (acetate)	anion-exchange ion chromatography	4.62	%	≤ 12.0	PASS
Residual solvent + inorganic residue	estimated — see /methods/residual-solvents-q3c/	0.40	%	—	NOT TESTED
Endotoxin	kinetic chromogenic LAL	2.51	EU/mg	≤ 5.0	PASS
Sterility	not ordered on this submission	not determined	—	no growth	NOT TESTED
Appearance	visual, diffuse light, black and white background	White lyophilised cake, intact	—	white to off-white intact cake	PASS
Reconstitution clarity	reconstituted per submitter procedure	Clear, colourless, no visible particulates	—	clear, no visible particulates	PASS
pH (reconstituted, 1 mg/mL)	potentiometric, two-point calibration	4.95	—	2.5 – 8.0	PASS
Retention time (observed)	RP-HPLC, 25 min gradient	7.95	min	7.38 ± 0.25 (normalised)	FAIL
Peak asymmetry (10 % height)	Ph. Eur. 2.2.46	1.01	—	0.80 – 1.50	PASS
Plate count, main peak	N = 5.54 (t/w½)²	79,814	—	≥ 20,000	PASS
Resolution to nearest impurity	vs deamidated (+1 Da)	1.59	—	no index criterion	REPORTED

Section 2

Chromatogram and peak integration

The figure is drawn from the table. It is not an independent illustration — if a number in the table changed, the trace would change with it.

Figure 1. RP-HPLC trace, 25 min gradient, 214 nm. Drawn from the peak-integration table below: peak positions are the reported retention times, peak areas are the reported area percentages, peak widths follow the reported plate count of 79,814, and skew follows the reported asymmetry factor of 1.01. Baseline noise is seeded from the report ID and is illustrative. The lower panel is the same trace at a stated vertical expansion with the slow gradient drift subtracted, because at full scale a 0.04 % impurity against a 99.93 % main peak is a line two pixels tall; the main peak runs off-scale there by design.

#	Peak	RT	Area (µV·s)	% area	Δ mass (Da)
1	ARA-290 — main peak	7.95	4,856,908	99.93	—
2	des-Gln truncate (−128 Da)	7.70	1,944	0.04	-128.059
3	deamidated (+1 Da)	7.77	1,458	0.03	+0.984
Closure check — purity + Σ impurities				100.00	CLOSES

Reporting threshold 0.05 % area. Peaks below it are not reported and are therefore excluded from the total, which overstates purity by their sum — see peak integration.

Section 3

Mass spectrum and identity

Theoretical monoisotopic mass 1189.6096 Da, computed from C50H83N15O18. All ppm figures on this site are against monoisotopic values — see why that matters.

Figure 2. ESI charge-state envelope, unit-resolution instrument. Sticks are placed at m/z = (M + z × 1.007276)/z for the observed neutral mass of 1189.6313 Da. Sodium (+21.982/z) and potassium (+37.956/z) adducts are shown where the parent charge state exceeds 25 % relative abundance; they are adducts of the same molecule and are not impurities.

Charge	Theoretical m/z	Observed m/z	Δ m/z	Rel. abundance (%)
3+	397.5438	397.5510	0.0072	8.8
2+	595.8121	595.8229	0.0108	52.9
1+	1190.6169	1190.6386	0.0217	100.0

Section 4

Gravimetric mass balance

Water, counter-ion, residual and total peptide content are exhaustive and mutually exclusive categories. They must account for 100 % of the cake mass. The index closes this to within 0.02 pp and the build asserts it.

Component	Determination	%w/w of cake
Water	Karl Fischer	4.90
Counter-ion (acetate)	ion chromatography	4.62
Residual solvent + inorganic residue	estimated	0.40
Total peptide content	by difference	90.08
Total		100.00 CLOSES

Supplier certificate, as declared

The supplier's own figures for this lot, beside the independent measurement. Divergence is assessed against the batch median, not against this single report — see rule AX-R05.

Figure	Supplier COA	This report	Index note
Purity (% area)	99.90	99.93	divergence -0.01 pp vs batch median
Total impurities (% area)	0.10	0.07
Largest single impurity (% area)	not stated	0.04
Water (%)	3.6	4.90
Counter-ion (%)	not stated	4.62
Peptide content (%w/w)	not stated	90.08
Endotoxin (EU/mg)	not stated	2.51
Method stated on certificate	YES	—	column, gradient and wavelength given

Section 5

Provenance and caveats

Where a sample came from changes the weight of the evidence. The index treats it as a first-class field.

Sample provenance: Buyer-sourced
Evidentiary weight: Strongest available evidence in this index.
Chain of custody: Retail purchase → submitter (ambient, 25 d) → laboratory (hand-carried, 5 d) → assay.
Assay date: 08 Jan 2026
Submitted to the index: 23 Jan 2026
Laboratory: Medutest, Ljubljana, Slovenia
Method basis: 25 min gradient · unit-resolution MS
Verification status: IDENTITY CONSISTENT

What this report does not establish

That the compound is not a near-isobaric alternative. Identity was confirmed at unit mass resolution, which cannot exclude species differing by 1 or 2 Da. The specific alternatives not excluded are named in the identity block above.
That the material is sterile. Sterility was not tested.
That any other vial in the same lot has the same composition. One vial was tested. Within-lot vial-to-vial variation in this index has a median of 0.28 pp and a 90th percentile of 0.74 pp.
That any other lot from the same supplier has the same composition. Batch-to-batch variation is larger than within-batch variation for every supplier here.
That the material is suitable for administration to a human or an animal. These are research compounds. No result on this site is a safety assessment and the index takes no position on use.
That the material was manufactured by the supplier that sold it. Contract synthesis is normal in this market and twenty-nine batches in the index fingerprint to a bulk from a different company than the one named on the label.

Section 6

Annotations 5

Signed technical comments from index contributors. Not a discussion forum — annotations question or contextualise the result, and the data team replies where a reply is warranted.

plate_count23 Jan 2026

Plate count on this run supports the separation claimed. A high purity figure from an inefficient column is a merged peak reported as a clean one; that is not what happened here.

coa_template23 Jan 2026

The certificate for WWB-2511-S3009 carries a named related-substances table rather than a totals line. That is what makes 99.93 % checkable against this record instead of merely comparable to it.

retest_date23 Jan 2026

A retest date on the certificate would tell you what the supplier is actually asserting about stability. Purity at 99.93 % on assay day is a starting condition, not a shelf life.

storage_stated23 Jan 2026

Stated storage conditions on the certificate against the observed transit conditions in the custody block: on this record they agree, which removes one class of explanation for anything unexpected.

valley_drop23 Jan 2026

Noted. Logged against the batch rollup.

Section 7

Related records

Same batch — lot WWB-2511-S3009 n=10

Report	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-041313	WWB-2511-S3009	Nordanalyt	2026-Q1	99.78	5.51	103.0	Buyer-sourced	0	PASS
AX-2026-041314	WWB-2511-S3009	PeptideMeter	2026-Q1	99.73	4.87	—	Laboratory-retained	0	PASS
AX-2026-041315	WWB-2511-S3009	PeptideMeter	2026-Q1	99.56	5.14	89.6	Buyer-sourced	1	MARGINAL
AX-2026-041316	WWB-2511-S3009	Janoshik	2026-Q1	99.92	4.34	104.4	Buyer-sourced	0	PASS
AX-2026-041317	WWB-2511-S3009	Janoshik	2026-Q1	99.93	4.08	—	Buyer-sourced	0	PASS
AX-2026-041319	WWB-2511-S3009	PeptideMeter	2026-Q1	99.93	5.52	99.5	Buyer-sourced	0	PASS
AX-2026-041320	WWB-2511-S3009	Janoshik	2026-Q1	99.70	5.13	—	Laboratory-retained	0	PASS
AX-2026-041321	WWB-2511-S3009	Janoshik	2026-Q1	99.92	4.28	—	Buyer-sourced	0	PASS
AX-2026-041322	WWB-2511-S3009	Janoshik	2026-Q1	99.90	5.59	107.2	Buyer-sourced	0	PASS

Batch rollup and reproducibility assessment →

Cross-sections

Other recent ARA-290 records in the index.
Report	Supplier	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Result
AX-2026-041767	ASY	ASY-{CAT}-1521	Janoshik	2026-Q3	99.66	4.42	103.7	Buyer-sourced	PASS
AX-2026-043222	SGN	SGN-2605-X9069	PeptideMeter	2026-Q2	98.32	7.36	—	Buyer-sourced	PASS
AX-2026-041907	SinoPep	SNP-2605-G4075	Janoshik	2026-Q2	99.20	5.75	—	Buyer-sourced	PASS
AX-2026-043227	SGN	SGN-2605-X9069	PeptideMeter	2026-Q2	98.38	7.21	95.6	Buyer-sourced	PASS
AX-2026-041862	GSP	GSP-2605-K3362	Janoshik	2026-Q2	99.34	3.32	—	Buyer-sourced	PASS
AX-2026-041906	SinoPep	SNP-2605-G4075	Medutest	2026-Q2	98.94	5.28	—	Buyer-sourced	PASS
AX-2026-042346	Homopeptide	HOMO-2601-T3123	PeptideMeter	2026-Q2	99.70	3.47	—	Buyer-sourced	PASS
AX-2026-043229	SGN	SGN-2605-X9069	Janoshik	2026-Q2	98.21	7.43	—	Buyer-sourced	PASS

Other recent WWB records in the index.
Report	Compound	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-041275	Liraglutide	WWB-2607-X2189	Nordanalyt	2026-Q3	99.33	4.39	—	Buyer-sourced	1	MARGINAL
AX-2026-041334	Semaglutide	WWB-2606-N3192	Janoshik	2026-Q3	98.93	4.62	99.9	Buyer-sourced	0	PASS
AX-2026-041337	Semaglutide	WWB-2606-N3192	Nordanalyt	2026-Q3	99.52	3.92	—	Buyer-sourced	0	PASS
AX-2026-041335	Semaglutide	WWB-2606-N3192	Janoshik	2026-Q3	99.15	4.27	98.9	Buyer-sourced	0	PASS
AX-2026-041271	Liraglutide	WWB-2607-X2189	Medutest	2026-Q3	99.52	4.57	97.4	Buyer-sourced	1	MARGINAL
AX-2026-041328	Semaglutide	WWB-2606-N3192	Janoshik	2026-Q3	98.99	4.47	106.1	Buyer-sourced	0	PASS
AX-2026-041333	Semaglutide	WWB-2606-N3192	Janoshik	2026-Q3	99.18	4.51	90.4	Laboratory-retained	0	PASS
AX-2026-041273	Liraglutide	WWB-2607-X2189	Janoshik	2026-Q3	99.31	5.52	104.8	Buyer-sourced	1	MARGINAL