Exenatide impurity profile
Every named species the index has reported alongside Exenatide, how often, at what level, and what its presence implies about the synthesis or the handling. Reporting threshold is 0.05 % area; anything below it is invisible to this page and is silently added to the main peak.
Median total impurities
1.02%
n=49
Median largest single
0.59%
n=49
Worst single observed
1.22%
n=49
Named species observed
5 / 5
n=5
| Named species | ΔRT (min) | Δ mass (Da) | Seen in | Median % area | Max % area |
|---|---|---|---|---|---|
| Met14 sulfoxide (+16 Da) | -0.88 | +15.995 | 98% | 0.18 | 1.01 |
| des-Gly1 truncate (−57 Da) | -0.42 | -57.021 | 96% | 0.10 | 0.66 |
| C-terminal free acid (+1 Da) | +0.34 | +0.984 | 96% | 0.15 | 0.96 |
| truncated (1-38) | -0.55 | -156.101 | 96% | 0.08 | 1.22 |
| Trp25 oxidation (+16 Da) | -0.71 | +15.995 | 90% | 0.10 | 0.91 |
Met14 sulfoxide (+16 Da)
- Retention offset from the main peak
- -0.88 min on RG-2
- Mass difference
- +15.995 Da
- Reported in
- 48 of 49 records (98 %)
- Level when reported
- median 0.18 %, max 1.01 %
- How it separates
- Baseline-resolved on the reference method.
des-Gly1 truncate (−57 Da)
- Retention offset from the main peak
- -0.42 min on RG-2
- Mass difference
- -57.021 Da
- Reported in
- 47 of 49 records (96 %)
- Level when reported
- median 0.10 %, max 0.66 %
- How it separates
- Baseline-resolved on the reference method.
C-terminal free acid (+1 Da)
- Retention offset from the main peak
- +0.34 min on RG-2
- Mass difference
- +0.984 Da
- Reported in
- 47 of 49 records (96 %)
- Level when reported
- median 0.15 %, max 0.96 %
- How it separates
- Baseline-resolved on the reference method.
truncated (1-38)
- Retention offset from the main peak
- -0.55 min on RG-2
- Mass difference
- -156.101 Da
- Reported in
- 47 of 49 records (96 %)
- Level when reported
- median 0.08 %, max 1.22 %
- How it separates
- Baseline-resolved on the reference method.
Trp25 oxidation (+16 Da)
- Retention offset from the main peak
- -0.71 min on RG-2
- Mass difference
- +15.995 Da
- Reported in
- 44 of 49 records (90 %)
- Level when reported
- median 0.10 %, max 0.91 %
- How it separates
- Baseline-resolved on the reference method.
Degradation pathways
Degradants are a handling finding rather than a synthesis finding. A lot that shipped clean and arrived oxidised tells you about the courier, not the chemist.
- Met14 oxidation — the dominant route, and the reason exenatide should be shipped cold
- C-terminal amide hydrolysis in acidic solution