NAD+ impurity profile
Every named species the index has reported alongside NAD+, how often, at what level, and what its presence implies about the synthesis or the handling. Reporting threshold is 0.05 % area; anything below it is invisible to this page and is silently added to the main peak.
Median total impurities
0.72%
n=44
Median largest single
0.48%
n=44
Worst single observed
1.13%
n=44
Named species observed
5 / 5
n=5
| Named species | ΔRT (min) | Δ mass (Da) | Seen in | Median % area | Max % area |
|---|---|---|---|---|---|
| nicotinamide mononucleotide (NMN, −331 Da) | -0.42 | -331.058 | 91% | 0.24 | 1.13 |
| α-NAD anomer | +0.18 | mass-identical | 91% | 0.13 | 0.90 |
| ADP-ribose (−121 Da) | -0.26 | -121.040 | 89% | 0.11 | 0.93 |
| nicotinamide (−541 Da) | -0.68 | -541.052 | 86% | 0.10 | 0.83 |
| NADH (reduced, +2 Da) | +0.34 | +2.016 | 86% | 0.11 | 0.43 |
nicotinamide mononucleotide (NMN, −331 Da)
- Retention offset from the main peak
- -0.42 min on RG-2
- Mass difference
- -331.058 Da
- Reported in
- 40 of 44 records (91 %)
- Level when reported
- median 0.24 %, max 1.13 %
- How it separates
- Baseline-resolved on the reference method.
α-NAD anomer
- Retention offset from the main peak
- +0.18 min on RG-2
- Mass difference
- mass-identical — chromatography only
- Reported in
- 40 of 44 records (91 %)
- Level when reported
- median 0.13 %, max 0.90 %
- How it separates
- Chromatography only. A gradient long enough to separate it; no mass spectrometer of any resolution will.
ADP-ribose (−121 Da)
- Retention offset from the main peak
- -0.26 min on RG-2
- Mass difference
- -121.040 Da
- Reported in
- 39 of 44 records (89 %)
- Level when reported
- median 0.11 %, max 0.93 %
- How it separates
- Close-eluting on the reference method, and merged with the main peak on a short gradient. The index prints the resolution figure for such a pair and applies no criterion to it — where the two are not separated, what matters is that the integration convention is stated.
nicotinamide (−541 Da)
- Retention offset from the main peak
- -0.68 min on RG-2
- Mass difference
- -541.052 Da
- Reported in
- 38 of 44 records (86 %)
- Level when reported
- median 0.10 %, max 0.83 %
- How it separates
- Baseline-resolved on the reference method.
NADH (reduced, +2 Da)
- Retention offset from the main peak
- +0.34 min on RG-2
- Mass difference
- +2.016 Da
- Reported in
- 38 of 44 records (86 %)
- Level when reported
- median 0.11 %, max 0.43 %
- How it separates
- Baseline-resolved on the reference method.
Degradation pathways
Degradants are a handling finding rather than a synthesis finding. A lot that shipped clean and arrived oxidised tells you about the courier, not the chemist.
- glycosidic hydrolysis to nicotinamide + ADP-ribose, fast above pH 8 and above 30 °C
- anomerisation to the inactive α form