AX-2025-019135 · NAD+ · GSP lot GSP-2507-A3926

Section 1

Results

Measured against the index acceptance specification for the non-peptide adjuncts class. A specification is a decision the index made; a result is a measurement. See where each threshold came from.

Parameter	Method	Result	Unit	Index spec
Purity	RP-HPLC, 35 min gradient, 214 nm	99.33	% area	≥ 97.0	PASS
Total impurities	RP-HPLC, sum of reported peaks	0.67	% area	≤ 3.0	PASS
Largest single impurity	RP-HPLC — nicotinamide mononucleotide (NMN, −331 Da)	0.24	% area	≤ 1.00	PASS
Identity — theoretical monoisotopic mass	calculated from molecular formula	663.1091	Da	—	NOT TESTED
Identity — observed monoisotopic mass	LC-MS, high resolution (ESI-TOF/QTOF)	663.0805	Da	—	NOT TESTED
Mass accuracy	observed vs theoretical	-43.1	ppm	± 10	FAIL
Total peptide content	by difference from the gravimetric mass balance	87.90	%w/w	—	NOT TESTED
Target-compound content	total peptide content × purity	87.31	%w/w	—	NOT TESTED
Gross fill (as weighed)	five-place balance, under nitrogen	109.19	mg	100 mg declared	NOT TESTED
Recovered target peptide	gross fill × target content	95.334	mg	100 mg claim	NOT TESTED
Recovery against label claim	recovered ÷ claim	95.3	%	90.0 – 110.0	PASS
Water content	Karl Fischer, coulometric, duplicate	11.67	%	≤ 10.0	FAIL
Counter-ion	no ionisable counter-ion expected	N/A	—	not applicable	NOT TESTED
Residual solvent + inorganic residue	estimated — see /methods/residual-solvents-q3c/	0.43	%	—	NOT TESTED
Endotoxin	not ordered on this submission	not determined	—	≤ 5.0	NOT TESTED
Sterility	not ordered on this submission	not determined	—	no growth	NOT TESTED
Appearance	visual, diffuse light, black and white background	White to pale-yellow cake, intact	—	white to off-white intact cake	PASS
Reconstitution clarity	reconstituted per submitter procedure	Clear, colourless, no visible particulates	—	clear, no visible particulates	PASS
pH (reconstituted, 1 mg/mL)	potentiometric, two-point calibration	7.24	—	2.5 – 8.0	PASS
Retention time (observed)	RP-HPLC, 35 min gradient	4.80	min	4.80 ± 0.25 (normalised)	PASS
Peak asymmetry (10 % height)	Ph. Eur. 2.2.46	1.15	—	0.80 – 1.50	PASS
Plate count, main peak	N = 5.54 (t/w½)²	68,145	—	≥ 20,000	PASS
Resolution to nearest impurity	vs α-NAD anomer	2.79	—	no index criterion	REPORTED

Section 2

Chromatogram and peak integration

The figure is drawn from the table. It is not an independent illustration — if a number in the table changed, the trace would change with it.

Figure 1. RP-HPLC trace, 35 min gradient, 214 nm. Drawn from the peak-integration table below: peak positions are the reported retention times, peak areas are the reported area percentages, peak widths follow the reported plate count of 68,145, and skew follows the reported asymmetry factor of 1.15. Baseline noise is seeded from the report ID and is illustrative. The lower panel is the same trace at a stated vertical expansion with the slow gradient drift subtracted, because at full scale a 0.24 % impurity against a 99.33 % main peak is a line two pixels tall; the main peak runs off-scale there by design.

#	Peak	RT	Area (µV·s)	% area	Δ mass (Da)
1	NAD+ — main peak	4.80	4,827,746	99.33	—
2	nicotinamide mononucleotide (NMN, −331 Da)	4.31	11,665	0.24	-331.058
3	nicotinamide (−541 Da)	4.01	10,207	0.21	-541.052
4	α-NAD anomer	5.01	6,318	0.13	0.000
5	NADH (reduced, +2 Da)	5.20	2,430	0.05	+2.016
6	ADP-ribose (−121 Da)	4.50	1,944	0.04	-121.040
Closure check — purity + Σ impurities				100.00	CLOSES

Reporting threshold 0.05 % area. Peaks below it are not reported and are therefore excluded from the total, which overstates purity by their sum — see peak integration.

Section 3

Mass spectrum and identity

Theoretical monoisotopic mass 663.1091 Da, computed from C21H27N7O14P2. All ppm figures on this site are against monoisotopic values — see why that matters.

Figure 2. ESI charge-state envelope, high-resolution instrument. Sticks are placed at m/z = (M + z × 1.007276)/z for the observed neutral mass of 663.0805 Da. Sodium (+21.982/z) and potassium (+37.956/z) adducts are shown where the parent charge state exceeds 25 % relative abundance; they are adducts of the same molecule and are not impurities.

Charge	Theoretical m/z	Observed m/z	Δ m/z	Rel. abundance (%)
2+	332.5618	332.5475	-0.0143	56.6
1+	664.1164	664.0878	-0.0286	100.0

Section 4

Gravimetric mass balance

Water, counter-ion, residual and total peptide content are exhaustive and mutually exclusive categories. They must account for 100 % of the cake mass. The index closes this to within 0.02 pp and the build asserts it.

Component	Determination	%w/w of cake
Water	Karl Fischer	11.67
Counter-ion	none expected	0.00
Residual solvent + inorganic residue	estimated	0.43
Total peptide content	by difference	87.90
Total		100.00 CLOSES

Supplier certificate, as declared

The supplier's own figures for this lot, beside the independent measurement. Divergence is assessed against the batch median, not against this single report — see rule AX-R05.

Figure	Supplier COA	This report	Index note
Purity (% area)	99.26	99.33	divergence +0.17 pp vs batch median
Total impurities (% area)	0.74	0.67
Largest single impurity (% area)	not stated	0.24
Water (%)	9.6	11.67
Counter-ion (%)	4.9	N/A
Peptide content (%w/w)	84.9	87.90
Endotoxin (EU/mg)	not stated	not determined
Method stated on certificate	YES	—	column, gradient and wavelength given

Section 5

Provenance and caveats

Where a sample came from changes the weight of the evidence. The index treats it as a first-class field.

Sample provenance: Buyer-sourced
Evidentiary weight: Strongest available evidence in this index.
Chain of custody: Retail purchase → submitter (−20 °C, 5 d) → laboratory (courier, dry ice, 11 d) → assay.
Assay date: 30 Jul 2025
Submitted to the index: 17 Aug 2025
Laboratory: Nordanalyt Laboratories, Uppsala, Sweden
Method basis: 35 min gradient · high-resolution MS
Verification status: PARTIAL

What this report does not establish

That the material is free of bacterial endotoxin. Endotoxin was not measured. No chromatographic result bears on it.
That the material is sterile. Sterility was not tested.
That any other vial in the same lot has the same composition. One vial was tested. Within-lot vial-to-vial variation in this index has a median of 0.28 pp and a 90th percentile of 0.74 pp.
That any other lot from the same supplier has the same composition. Batch-to-batch variation is larger than within-batch variation for every supplier here.
That the material is suitable for administration to a human or an animal. These are research compounds. No result on this site is a safety assessment and the index takes no position on use.
That the material was manufactured by the supplier that sold it. Contract synthesis is normal in this market and twenty-nine batches in the index fingerprint to a bulk from a different company than the one named on the label.

Section 6

Annotations 4

Signed technical comments from index contributors. Not a discussion forum — annotations question or contextualise the result, and the data team replies where a reply is warranted.

gradient_2517 Aug 2025

The gradient is on the record, which is the first thing that should be true of any purity figure and often is not. 35 min at Nordanalyt puts this comparison on a documented footing.

buyer_sourced17 Aug 2025

Buyer-sourced through an ordinary retail order, so the supplier had no opportunity to select the vial. This is the provenance class the index weights and it is roughly 87 % of the dataset.

bland_altman17 Aug 2025

Where two laboratories tested the same retail lot, the paired difference is a clean estimate of inter-lab bias with no selection term in it at all. Those pairs are the backbone of the agreement analysis.

iso_audit17 Aug 2025

A certification says what a process is supposed to do. A retail vial says what it did. Both are worth having and only one of them is a measurement.

Section 7

Related records

Same batch — lot GSP-2507-A3926 n=2

Report	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2025-019134	GSP-2507-A3926	Janoshik	2025-Q3	98.84	11.68	—	Buyer-sourced	0	FAIL

Batch rollup and reproducibility assessment →

Cross-sections

Other recent NAD+ records in the index.
Report	Supplier	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-041208	WWB	WWB-2601-V1583	VendorInvestigate	2026-Q2	99.47	11.38	100.9	Buyer-sourced	0	FAIL
AX-2026-041209	WWB	WWB-2601-V1583	Nordanalyt	2026-Q2	99.47	11.40	106.7	Buyer-sourced	0	FAIL
AX-2026-041210	WWB	WWB-2601-V1583	Nordanalyt	2026-Q2	99.66	11.01	91.9	Buyer-sourced	0	FAIL
AX-2026-041211	WWB	WWB-2601-V1583	Janoshik	2026-Q2	99.72	10.94	93.5	Buyer-sourced	0	FAIL
AX-2026-041213	WWB	WWB-2601-V1583	VendorInvestigate	2026-Q2	99.33	11.41	—	Buyer-sourced	0	FAIL
AX-2026-041212	WWB	WWB-2601-V1583	Janoshik	2026-Q2	99.93	10.36	88.5	Laboratory-retained	1	FAIL
AX-2025-019628	TFC	TFC-2505-C1792	Janoshik	2025-Q3	99.72	11.19	92.6	Buyer-sourced	0	FAIL
AX-2025-019134	GSP	GSP-2507-A3926	Janoshik	2025-Q3	98.84	11.68	—	Buyer-sourced	0	FAIL

Other recent GSP records in the index.
Report	Compound	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-041831	Survodutide	GSP-2603-E3156	Kestrel	2026-Q3	99.91	5.50	93.2	Buyer-sourced	0	PASS
AX-2026-041836	Survodutide	GSP-2603-E3156	Janoshik	2026-Q3	98.65	5.34	90.3	Buyer-sourced	0	PASS
AX-2026-041837	Survodutide	GSP-2603-E3156	Janoshik	2026-Q3	98.33	5.37	—	Buyer-sourced	0	PASS
AX-2026-041833	Survodutide	GSP-2603-E3156	Janoshik	2026-Q3	98.08	4.91	99.3	Buyer-sourced	0	PASS
AX-2026-041832	Survodutide	GSP-2603-E3156	VendorInvestigate	2026-Q3	98.01	5.14	99.5	Buyer-sourced	0	PASS
AX-2026-041835	Survodutide	GSP-2603-E3156	Nordanalyt	2026-Q3	98.34	5.74	89.0	Buyer-sourced	1	MARGINAL
AX-2026-041834	Survodutide	GSP-2603-E3156	VendorInvestigate	2026-Q3	97.92	5.95	—	Buyer-sourced	0	PASS
AX-2026-041838	Survodutide	GSP-2603-E3156	PeptideMeter	2026-Q3	98.30	5.01	102.4	Buyer-sourced	0	PASS