AX-2025-020775 · Adipotide · CPC lot CPC-2502-C9355

Section 1

Results

Measured against the index acceptance specification for the metabolic and mitochondrial peptides class. A specification is a decision the index made; a result is a measurement. See where each threshold came from.

Parameter	Method	Result	Unit	Index spec
Purity	RP-HPLC, 25 min gradient, 214 nm	97.48	% area	≥ 96.0	PASS
Total impurities	RP-HPLC, sum of reported peaks	2.52	% area	≤ 4.0	PASS
Largest single impurity	RP-HPLC — truncated KLAKLAK repeat (−781 Da)	1.75	% area	≤ 1.00	FAIL
Identity — theoretical monoisotopic mass	calculated from molecular formula	4095.6238	Da	—	NOT TESTED
Identity — observed monoisotopic mass	LC-MS, unit resolution (single quadrupole)	4095.7090	Da	—	NOT TESTED
Mass accuracy	observed vs theoretical	+20.8	ppm	± 15	FAIL
Total peptide content	by difference from the gravimetric mass balance	88.65	%w/w	—	NOT TESTED
Target-compound content	total peptide content × purity	86.42	%w/w	—	NOT TESTED
Gross fill (as weighed)	five-place balance, under nitrogen	2.38	mg	2 mg declared	NOT TESTED
Recovered target peptide	gross fill × target content	2.057	mg	2 mg claim	NOT TESTED
Recovery against label claim	recovered ÷ claim	102.9	%	90.0 – 110.0	PASS
Water content	Karl Fischer, coulometric, duplicate	5.21	%	≤ 9.0	PASS
Counter-ion (TFA)	anion-exchange ion chromatography	5.12	%	≤ 12.0	PASS
Residual solvent + inorganic residue	estimated — see /methods/residual-solvents-q3c/	1.02	%	—	NOT TESTED
Endotoxin	not ordered on this submission	not determined	—	≤ 5.0	NOT TESTED
Sterility	not ordered on this submission	not determined	—	no growth	NOT TESTED
Appearance	visual, diffuse light, black and white background	Off-white lyophilised cake, intact	—	white to off-white intact cake	PASS
Reconstitution clarity	reconstituted per submitter procedure	Clear, colourless, no visible particulates	—	clear, no visible particulates	PASS
pH (reconstituted, 1 mg/mL)	potentiometric, two-point calibration	3.76	—	2.5 – 8.0	PASS
Retention time (observed)	RP-HPLC, 25 min gradient	10.88	min	10.79 ± 0.35 (normalised)	PASS
Peak asymmetry (10 % height)	Ph. Eur. 2.2.46	1.21	—	0.80 – 1.50	PASS
Plate count, main peak	N = 5.54 (t/w½)²	69,433	—	≥ 20,000	PASS
Resolution to nearest impurity	vs disulfide-scrambled isomer	2.17	—	no index criterion	REPORTED

Section 2

Chromatogram and peak integration

The figure is drawn from the table. It is not an independent illustration — if a number in the table changed, the trace would change with it.

Figure 1. RP-HPLC trace, 25 min gradient, 214 nm. Drawn from the peak-integration table below: peak positions are the reported retention times, peak areas are the reported area percentages, peak widths follow the reported plate count of 69,433, and skew follows the reported asymmetry factor of 1.21. Baseline noise is seeded from the report ID and is illustrative. The lower panel is the same trace at a stated vertical expansion with the slow gradient drift subtracted, because at full scale a 1.75 % impurity against a 97.48 % main peak is a line two pixels tall; the main peak runs off-scale there by design.

#	Peak	RT	Area (µV·s)	% area	Δ mass (Da)
1	Adipotide — main peak	10.88	4,737,830	97.48	—
2	truncated KLAKLAK repeat (−781 Da)	9.53	85,055	1.75	-781.512
3	disulfide-scrambled isomer	11.25	24,788	0.51	0.000
4	open-chain (reduced disulfide, +2 Da)	10.01	7,776	0.16	+2.016
5	trifluoroacetyl adduct (+96 Da)	11.56	4,860	0.10	+95.982
Closure check — purity + Σ impurities				100.00	CLOSES

Reporting threshold 0.05 % area. Peaks below it are not reported and are therefore excluded from the total, which overstates purity by their sum — see peak integration.

Section 3

Mass spectrum and identity

Theoretical monoisotopic mass 4095.6238 Da, computed from C190H332N60O43. All ppm figures on this site are against monoisotopic values — see why that matters.

Figure 2. ESI charge-state envelope, unit-resolution instrument. Sticks are placed at m/z = (M + z × 1.007276)/z for the observed neutral mass of 4095.7090 Da. Sodium (+21.982/z) and potassium (+37.956/z) adducts are shown where the parent charge state exceeds 25 % relative abundance; they are adducts of the same molecule and are not impurities.

Charge	Theoretical m/z	Observed m/z	Δ m/z	Rel. abundance (%)
6+	683.6112	683.6254	0.0142	7.7
5+	820.1320	820.1491	0.0171	52.5
4+	1024.9132	1024.9345	0.0213	100.0
3+	1366.2152	1366.2436	0.0284	51.6
2+	2048.8192	2048.8618	0.0426	8.4

Section 4

Gravimetric mass balance

Water, counter-ion, residual and total peptide content are exhaustive and mutually exclusive categories. They must account for 100 % of the cake mass. The index closes this to within 0.02 pp and the build asserts it.

Component	Determination	%w/w of cake
Water	Karl Fischer	5.21
Counter-ion (TFA)	ion chromatography	5.12
Residual solvent + inorganic residue	estimated	1.02
Total peptide content	by difference	88.65
Total		100.00 CLOSES

Supplier certificate, as declared

The supplier's own figures for this lot, beside the independent measurement. Divergence is assessed against the batch median, not against this single report — see rule AX-R05.

Figure	Supplier COA	This report	Index note
Purity (% area)	97.61	97.48	divergence +0.13 pp vs batch median
Total impurities (% area)	not stated	2.52
Largest single impurity (% area)	not stated	1.75
Water (%)	not stated	5.21
Counter-ion (%)	not stated	5.12
Peptide content (%w/w)	91.7	88.65
Endotoxin (EU/mg)	not stated	not determined
Method stated on certificate	YES	—	column, gradient and wavelength given

Section 5

Provenance and caveats

Where a sample came from changes the weight of the evidence. The index treats it as a first-class field.

Sample provenance: Buyer-sourced
Evidentiary weight: Strongest available evidence in this index.
Chain of custody: Domestic warehouse despatch → submitter (2–8 °C, 18 d) → laboratory (courier, cold pack, 7 d) → assay.
Assay date: 25 Apr 2025
Submitted to the index: 18 May 2025
Laboratory: Medutest, Ljubljana, Slovenia
Method basis: 25 min gradient · unit-resolution MS
Verification status: IDENTITY CONSISTENT

What this report does not establish

That the compound is not a near-isobaric alternative. Identity was confirmed at unit mass resolution, which cannot exclude species differing by 1 or 2 Da. The specific alternatives not excluded are named in the identity block above.
That the material is free of bacterial endotoxin. Endotoxin was not measured. No chromatographic result bears on it.
That the material is sterile. Sterility was not tested.
That any other vial in the same lot has the same composition. One vial was tested. Within-lot vial-to-vial variation in this index has a median of 0.28 pp and a 90th percentile of 0.74 pp.
That any other lot from the same supplier has the same composition. Batch-to-batch variation is larger than within-batch variation for every supplier here.
That the material is suitable for administration to a human or an animal. These are research compounds. No result on this site is a safety assessment and the index takes no position on use.
That the material was manufactured by the supplier that sold it. Contract synthesis is normal in this market and twenty-nine batches in the index fingerprint to a bulk from a different company than the one named on the label.

Section 6

Annotations 3

Signed technical comments from index contributors. Not a discussion forum — annotations question or contextualise the result, and the data team replies where a reply is warranted.

units_pedant18 May 2025

Retention at 10.88 min is consistent with Adipotide, and retention is an independent constraint on identity. A mass error of +20.8 ppm against a correct retention time points at the measurement rather than at the vial.

ms_confirm18 May 2025

Nothing about +20.8 ppm bears on the purity figure on this record. Chromatographic homogeneity and mass accuracy are independent measurements and only one of them is in question here.

buyer_sourced18 May 2025

Independently purchased, at the submitter's own cost, forwarded without the supplier's involvement. That is the definition and this record meets it.

Section 7

Related records

Same batch — lot CPC-2502-C9355 n=3

Report	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2025-020773	CPC-2502-C9355	Medutest	2025-Q2	97.28	5.78	—	Buyer-sourced	0	PASS
AX-2025-020774	CPC-2502-C9355	VendorInvestigate	2025-Q2	97.50	5.68	—	Buyer-sourced	0	PASS

Batch rollup and reproducibility assessment →

Cross-sections

Other recent Adipotide records in the index.
Report	Supplier	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Result
AX-2026-043019	MKM	MKM-2606-K5012	Janoshik	2026-Q3	97.61	5.77	105.1	Buyer-sourced	PASS
AX-2026-043020	MKM	MKM-2606-K5012	Medutest	2026-Q3	97.73	5.74	—	Buyer-sourced	PASS
AX-2026-043022	MKM	MKM-2606-K5012	Kestrel	2026-Q3	97.69	6.33	106.9	Buyer-sourced	PASS
AX-2026-043023	MKM	MKM-2606-K5012	Medutest	2026-Q3	97.48	5.56	103.7	Supplier-supplied	PASS
AX-2026-043024	MKM	MKM-2606-K5012	Kestrel	2026-Q3	98.61	5.07	—	Buyer-sourced	PASS
AX-2026-043021	MKM	MKM-2606-K5012	VendorInvestigate	2026-Q3	97.74	5.83	96.9	Buyer-sourced	PASS
AX-2026-043567	SIG	SIG-2603-6617	Janoshik	2026-Q2	98.54	3.08	96.6	Buyer-sourced	PASS
AX-2026-043566	SIG	SIG-2603-6617	PeptideMeter	2026-Q2	98.26	2.86	103.1	Buyer-sourced	PASS

Other recent CPC records in the index.
Report	Compound	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-043319	BPC-157	CPC-2602-W1610	Medutest	2026-Q3	98.82	2.88	103.5	Buyer-sourced	2	MARGINAL
AX-2026-043295	Liraglutide	CPC-2602-G9942	Janoshik	2026-Q3	97.34	5.17	106.3	Buyer-sourced	0	FAIL
AX-2026-043288	Tirzepatide	CPC-2607-L1516	Janoshik	2026-Q3	96.89	5.11	101.1	Laboratory-retained	0	FAIL
AX-2026-043327	N-Acetyl Semax Amide	CPC-2602-D1905	Nordanalyt	2026-Q3	99.05	5.67	90.6	Buyer-sourced	1	FAIL
AX-2026-043328	N-Acetyl Semax Amide	CPC-2602-D1905	Janoshik	2026-Q3	99.49	6.83	100.9	Supplier-supplied	0	PASS
AX-2026-043257	L-Carnosine	CPC-2603-G9615	VendorInvestigate	2026-Q3	98.20	10.41	102.2	Buyer-sourced	0	FAIL
AX-2026-043297	Liraglutide	CPC-2602-G9942	Janoshik	2026-Q3	97.01	4.95	103.5	Buyer-sourced	0	FAIL
AX-2026-043331	N-Acetyl Semax Amide	CPC-2602-D1905	Janoshik	2026-Q3	99.46	6.22	—	Supplier-supplied	0	PASS