AssayIndex
Cross-section

LL-37 by laboratory

The same compound measured by different laboratories on different gradients. Where the medians differ, the question is whether the material differed or the method did — and for LL-37, with a class minimum gradient of 15 min, the method is a live explanation.

17 recordsmedian 98.90 %spec 96.5 %
LaboratoryReportsGradientMS resolutionMedian purity %Published bias (pp)Bias-adjustedMethod suitability
Janoshik830minHigh99.09+0.0099.09ADEQUATE
PeptideMeter3 n=328minHigh98.93-0.0598.98ADEQUATE
Medutest2 n=225minUnit99.30, 98.80+0.08—ADEQUATE
VendorInvestigate2 n=232minHigh98.12, 98.19-0.11—ADEQUATE
Nordanalyt2 n=235minHigh98.75, 98.90+0.02—ADEQUATE
Kestrel015minUnit—+0.24—ADEQUATE
97.998.298.598.899.299.599.8JanoshikJanoshik: Q1 98.62, median 99.09, Q3 99.38, n=8n=8PeptideMeterPeptideMeter: Q1 98.50, median 98.93, Q3 99.12, n=3n=3MedutestMedutest: Q1 98.92, median 99.05, Q3 99.17, n=2n=2VendorInvestigateVendorInvestigate: Q1 98.14, median 98.16, Q3 98.17, n=2n=2NordanalytNordanalyt: Q1 98.79, median 98.83, Q3 98.86, n=2n=2 Purity (% area)
Figure. LL-37 purity by contributing laboratory. Any offset between rows has two possible sources — a real difference in the material each laboratory happened to receive, and the laboratory's own bias — and this figure cannot separate them. The paired analysis that can is on inter-lab agreement.