AX-2024-004638

AOD-9604 · 10 mg declared · Tianjin Finder Chemical Co., Ltd. · lot TFC-2402-L2242

PASSVERIFIED

Report ID: AX-2024-004638
Compound: AOD-9604
Compound class: Metabolic and mitochondrial peptides
Supplier: TFC — Tianjin Finder Chemical Co., Ltd.
Batch / lot: TFC-2402-L2242
Declared label claim: 10mg
Laboratory: VendorInvestigate
Assay date: 23 Apr 2024
Submitted: 15 May 2024
Quarter: 2024-Q2
Submitter: ke.dahlberg
Sample provenance: Buyer-sourced

Section 1

Results

Measured against the index acceptance specification for the metabolic and mitochondrial peptides class. A specification is a decision the index made; a result is a measurement. See where each threshold came from.

Parameter	Method	Result	Unit	Index spec
Purity	RP-HPLC, 32 min gradient, 214 nm	99.19	% area	≥ 98.0	PASS
Total impurities	RP-HPLC, sum of reported peaks	0.81	% area	≤ 2.0	PASS
Largest single impurity	RP-HPLC — AOD dimer	0.33	% area	≤ 1.00	PASS
Identity — theoretical monoisotopic mass	calculated from molecular formula	1815.8672	Da	—	NOT TESTED
Identity — observed monoisotopic mass	LC-MS, high resolution (ESI-TOF/QTOF)	1815.8710	Da	—	NOT TESTED
Mass accuracy	observed vs theoretical	+2.1	ppm	± 15	PASS
Total peptide content	by difference from the gravimetric mass balance	88.08	%w/w	—	NOT TESTED
Target-compound content	total peptide content × purity	87.37	%w/w	—	NOT TESTED
Gross fill (as weighed)	five-place balance, under nitrogen	11.46	mg	10 mg declared	NOT TESTED
Recovered target peptide	gross fill × target content	10.013	mg	10 mg claim	NOT TESTED
Recovery against label claim	recovered ÷ claim	100.1	%	90.0 – 110.0	PASS
Water content	Karl Fischer, coulometric, duplicate	5.89	%	≤ 9.0	PASS
Counter-ion (acetate)	anion-exchange ion chromatography	5.50	%	≤ 12.0	PASS
Residual solvent + inorganic residue	estimated — see /methods/residual-solvents-q3c/	0.53	%	—	NOT TESTED
Endotoxin	kinetic chromogenic LAL	0.25	EU/mg	≤ 5.0	PASS
Sterility	membrane filtration, USP <71>, 14 d	No growth at 14 d	—	no growth	PASS
Appearance	visual, diffuse light, black and white background	White cake, partially collapsed	—	white to off-white intact cake	FAIL
Reconstitution clarity	reconstituted per submitter procedure	Clear, colourless, no visible particulates	—	clear, no visible particulates	PASS
pH (reconstituted, 1 mg/mL)	potentiometric, two-point calibration	5.06	—	2.5 – 8.0	PASS
Retention time (observed)	RP-HPLC, 32 min gradient	12.10	min	11.97 ± 0.30 (normalised)	PASS
Peak asymmetry (10 % height)	Ph. Eur. 2.2.46	1.01	—	0.80 – 1.50	PASS
Plate count, main peak	N = 5.54 (t/w½)²	47,919	—	≥ 20,000	PASS
Resolution to nearest impurity	vs Met sulfoxide (+16 Da)	2.07	—	no index criterion	REPORTED

Section 2

Chromatogram and peak integration

The figure is drawn from the table. It is not an independent illustration — if a number in the table changed, the trace would change with it.

Figure 1. RP-HPLC trace, 32 min gradient, 214 nm. Drawn from the peak-integration table below: peak positions are the reported retention times, peak areas are the reported area percentages, peak widths follow the reported plate count of 47,919, and skew follows the reported asymmetry factor of 1.01. Baseline noise is seeded from the report ID and is illustrative. The lower panel is the same trace at a stated vertical expansion with the slow gradient drift subtracted, because at full scale a 0.33 % impurity against a 99.19 % main peak is a line two pixels tall; the main peak runs off-scale there by design.

#	Peak	RT	Area (µV·s)	% area	Δ mass (Da)
1	AOD-9604 — main peak	12.10	4,820,941	99.19	—
2	AOD dimer	14.34	16,039	0.33	+1813.852
3	open-chain (reduced disulfide, +2 Da)	11.45	10,207	0.21	+2.016
4	Met sulfoxide (+16 Da)	11.63	6,804	0.14	+15.995
5	des-Tyr AOD (−163 Da)	11.20	6,318	0.13	-163.063
Closure check — purity + Σ impurities				100.00	CLOSES

Reporting threshold 0.05 % area. Peaks below it are not reported and are therefore excluded from the total, which overstates purity by their sum — see peak integration.

Section 3

Mass spectrum and identity

Theoretical monoisotopic mass 1815.8672 Da, computed from C78H123N23O23S2. All ppm figures on this site are against monoisotopic values — see why that matters.

Figure 2. ESI charge-state envelope, high-resolution instrument. Sticks are placed at m/z = (M + z × 1.007276)/z for the observed neutral mass of 1815.8710 Da. Sodium (+21.982/z) and potassium (+37.956/z) adducts are shown where the parent charge state exceeds 25 % relative abundance; they are adducts of the same molecule and are not impurities.

Charge	Theoretical m/z	Observed m/z	Δ m/z	Rel. abundance (%)
4+	454.9741	454.9750	0.0009	8.1
3+	606.2963	606.2976	0.0013	59.1
2+	908.9409	908.9428	0.0019	100.0
1+	1816.8745	1816.8783	0.0038	59.4

Section 4

Gravimetric mass balance

Water, counter-ion, residual and total peptide content are exhaustive and mutually exclusive categories. They must account for 100 % of the cake mass. The index closes this to within 0.02 pp and the build asserts it.

Component	Determination	%w/w of cake
Water	Karl Fischer	5.89
Counter-ion (acetate)	ion chromatography	5.50
Residual solvent + inorganic residue	estimated	0.53
Total peptide content	by difference	88.08
Total		100.00 CLOSES

Supplier certificate, as declared

The supplier's own figures for this lot, beside the independent measurement. Divergence is assessed against the batch median, not against this single report — see rule AX-R05.

Figure	Supplier COA	This report	Index note
Purity (% area)	98.77	99.19	divergence -0.42 pp vs batch median
Total impurities (% area)	1.23	0.81
Largest single impurity (% area)	0.47	0.33
Water (%)	3.2	5.89
Counter-ion (%)	4.9	5.50
Peptide content (%w/w)	91.3	88.08
Endotoxin (EU/mg)	0.20	0.25
Method stated on certificate	NO	—	no method section — the result cannot be reconciled against any other

Section 5

Provenance and caveats

Where a sample came from changes the weight of the evidence. The index treats it as a first-class field.

Sample provenance: Buyer-sourced
Evidentiary weight: Strongest available evidence in this index.
Chain of custody: Domestic warehouse despatch → submitter (2–8 °C, 28 d) → laboratory (courier, cold pack, 2 d) → assay.
Assay date: 23 Apr 2024
Submitted to the index: 15 May 2024
Laboratory: VendorInvestigate, Riga, Latvia
Method basis: 32 min gradient · high-resolution MS
Verification status: VERIFIED

Section 6

Annotations

No annotations on this record. Annotations are short signed technical comments from index contributors; see how to contribute.

Section 7

Related records

Same batch — lot TFC-2402-L2242 n=5

Report	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2024-004634	TFC-2402-L2242	Janoshik	2024-Q2	99.11	5.36	—	Buyer-sourced	0	PASS
AX-2024-004635	TFC-2402-L2242	Janoshik	2024-Q2	99.27	5.62	—	Buyer-sourced	0	PASS
AX-2024-004636	TFC-2402-L2242	Kestrel	2024-Q2	99.77	5.69	88.7	Buyer-sourced	1	MARGINAL
AX-2024-004637	TFC-2402-L2242	Janoshik	2024-Q2	99.19	5.09	—	Buyer-sourced	1	MARGINAL

Batch rollup and reproducibility assessment →

Cross-sections

Other recent AOD-9604 records in the index.
Report	Supplier	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Result
AX-2026-043668	PPG	PPG-2602-W8018	PeptideMeter	2026-Q2	99.47	3.06	—	Buyer-sourced	PASS
AX-2026-043670	PPG	PPG-2602-W8018	PeptideMeter	2026-Q2	99.56	2.71	104.5	Buyer-sourced	PASS
AX-2026-042545	HJ	HJ-2601-N5554	Nordanalyt	2026-Q2	98.84	5.56	90.8	Buyer-sourced	PASS
AX-2026-042540	HJ	HJ-2601-N5554	Janoshik	2026-Q2	99.08	5.58	93.6	Buyer-sourced	PASS
AX-2026-043669	PPG	PPG-2602-W8018	Janoshik	2026-Q2	99.63	2.43	97.0	Buyer-sourced	PASS
AX-2026-043671	PPG	PPG-2602-W8018	PeptideMeter	2026-Q2	98.75	2.10	93.4	Buyer-sourced	PASS
AX-2026-042544	HJ	HJ-2601-N5554	Janoshik	2026-Q2	99.36	5.12	93.2	Supplier-supplied	PASS
AX-2026-042543	HJ	HJ-2601-N5554	PeptideMeter	2026-Q2	98.93	5.67	104.5	Laboratory-retained	PASS

Other recent TFC records in the index.
Report	Compound	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-042265	Humanin (HNG)	TFC-2605-U1300	PeptideMeter	2026-Q3	99.52	6.31	—	Buyer-sourced	0	PASS
AX-2026-042222	SS-31 (Elamipretide)	TFC-2607-R9099	VendorInvestigate	2026-Q3	99.46	5.41	102.6	Buyer-sourced	0	PASS
AX-2026-042236	Humanin (HNG)	TFC-2608-S9940	Nordanalyt	2026-Q3	98.64	1.80	—	Buyer-sourced	0	PASS
AX-2026-042282	Retatrutide	TFC-2608-J1156	Janoshik	2026-Q3	98.54	4.27	—	Buyer-sourced	0	PASS
AX-2026-042226	SS-31 (Elamipretide)	TFC-2607-R9099	Janoshik	2026-Q3	99.62	5.47	—	Buyer-sourced	0	PASS
AX-2026-042217	Semaglutide	TFC-2604-X9037	PeptideMeter	2026-Q3	98.95	4.61	96.9	Buyer-sourced	0	PASS
AX-2026-042339	Sermorelin	TFC-2605-Y2176	Kestrel	2026-Q3	99.93	4.14	—	Supplier-supplied	1	FAIL
AX-2026-042214	Semaglutide	TFC-2604-X9037	Kestrel	2026-Q3	99.87	4.66	103.1	Laboratory-retained	0	PASS