AX-2025-018532 · GHRP-2 · QYB lot QYB-2510-D4215

Section 1

Results

Measured against the index acceptance specification for the growth-hormone axis peptides class. A specification is a decision the index made; a result is a measurement. See where each threshold came from.

Parameter	Method	Result	Unit	Index spec
Purity	RP-HPLC, 28 min gradient, 214 nm	99.30	% area	≥ 99.0	PASS
Total impurities	RP-HPLC, sum of reported peaks	0.70	% area	≤ 1.0	PASS
Largest single impurity	RP-HPLC — oxidised Trp (+16 Da)	0.28	% area	≤ 1.00	PASS
Identity — theoretical monoisotopic mass	calculated from molecular formula	817.4275	Da	—	NOT TESTED
Identity — observed monoisotopic mass	LC-MS, high resolution (ESI-TOF/QTOF)	817.4294	Da	—	NOT TESTED
Mass accuracy	observed vs theoretical	+2.3	ppm	± 15	PASS
Total peptide content	by difference from the gravimetric mass balance	90.70	%w/w	—	NOT TESTED
Target-compound content	total peptide content × purity	90.07	%w/w	—	NOT TESTED
Gross fill (as weighed)	five-place balance, under nitrogen	4.93	mg	5 mg declared	NOT TESTED
Recovered target peptide	gross fill × target content	4.440	mg	5 mg claim	NOT TESTED
Recovery against label claim	recovered ÷ claim	88.8	%	90.0 – 110.0	FAIL
Water content	Karl Fischer, coulometric, duplicate	4.26	%	≤ 9.0	PASS
Counter-ion (acetate)	anion-exchange ion chromatography	4.90	%	≤ 12.0	PASS
Residual solvent + inorganic residue	estimated — see /methods/residual-solvents-q3c/	0.14	%	—	NOT TESTED
Endotoxin	not ordered on this submission	not determined	—	≤ 5.0	NOT TESTED
Sterility	not ordered on this submission	not determined	—	no growth	NOT TESTED
Appearance	visual, diffuse light, black and white background	Off-white lyophilised cake, intact	—	white to off-white intact cake	PASS
Reconstitution clarity	reconstituted per submitter procedure	Clear with a single visible fibre	—	clear, no visible particulates	FAIL
pH (reconstituted, 1 mg/mL)	potentiometric, two-point calibration	4.80	—	2.5 – 8.0	PASS
Retention time (observed)	RP-HPLC, 28 min gradient	8.62	min	8.50 ± 0.25 (normalised)	PASS
Peak asymmetry (10 % height)	Ph. Eur. 2.2.46	1.00	—	0.80 – 1.50	PASS
Plate count, main peak	N = 5.54 (t/w½)²	66,941	—	≥ 20,000	PASS
Resolution to nearest impurity	vs [D-2-Nal]-epimer	0.96	—	no index criterion	REPORTED

Section 2

Chromatogram and peak integration

The figure is drawn from the table. It is not an independent illustration — if a number in the table changed, the trace would change with it.

Figure 1. RP-HPLC trace, 28 min gradient, 214 nm. Drawn from the peak-integration table below: peak positions are the reported retention times, peak areas are the reported area percentages, peak widths follow the reported plate count of 66,941, and skew follows the reported asymmetry factor of 1.00. Baseline noise is seeded from the report ID and is illustrative. The lower panel is the same trace at a stated vertical expansion with the slow gradient drift subtracted, because at full scale a 0.28 % impurity against a 99.30 % main peak is a line two pixels tall; the main peak runs off-scale there by design.

#	Peak	RT	Area (µV·s)	% area	Δ mass (Da)
1	GHRP-2 — main peak	8.62	4,826,288	99.30	—
2	oxidised Trp (+16 Da)	8.28	13,609	0.28	+15.995
3	C-terminal free acid (+1 Da)	8.77	10,207	0.21	+0.984
4	[D-2-Nal]-epimer	8.49	6,318	0.13	0.000
5	des-Lys (−128 Da)	8.40	3,888	0.08	-128.095
Closure check — purity + Σ impurities				100.00	CLOSES

Reporting threshold 0.05 % area. Peaks below it are not reported and are therefore excluded from the total, which overstates purity by their sum — see peak integration.

Section 3

Mass spectrum and identity

Theoretical monoisotopic mass 817.4275 Da, computed from C45H55N9O6. All ppm figures on this site are against monoisotopic values — see why that matters.

Figure 2. ESI charge-state envelope, high-resolution instrument. Sticks are placed at m/z = (M + z × 1.007276)/z for the observed neutral mass of 817.4294 Da. Sodium (+21.982/z) and potassium (+37.956/z) adducts are shown where the parent charge state exceeds 25 % relative abundance; they are adducts of the same molecule and are not impurities.

Charge	Theoretical m/z	Observed m/z	Δ m/z	Rel. abundance (%)
2+	409.7210	409.7220	0.0010	56.3
1+	818.4348	818.4367	0.0019	100.0

Section 4

Gravimetric mass balance

Water, counter-ion, residual and total peptide content are exhaustive and mutually exclusive categories. They must account for 100 % of the cake mass. The index closes this to within 0.02 pp and the build asserts it.

Component	Determination	%w/w of cake
Water	Karl Fischer	4.26
Counter-ion (acetate)	ion chromatography	4.90
Residual solvent + inorganic residue	estimated	0.14
Total peptide content	by difference	90.70
Total		100.00 CLOSES

Supplier certificate, as declared

The supplier's own figures for this lot, beside the independent measurement. Divergence is assessed against the batch median, not against this single report — see rule AX-R05.

Figure	Supplier COA	This report	Index note
Purity (% area)	98.78	99.30	divergence -0.27 pp vs batch median
Total impurities (% area)	not stated	0.70
Largest single impurity (% area)	not stated	0.28
Water (%)	1.9	4.26
Counter-ion (%)	not stated	4.90
Peptide content (%w/w)	not stated	90.70
Endotoxin (EU/mg)	not stated	not determined
Method stated on certificate	YES	—	column, gradient and wavelength given

Section 5

Provenance and caveats

Where a sample came from changes the weight of the evidence. The index treats it as a first-class field.

Sample provenance: Buyer-sourced
Evidentiary weight: Strongest available evidence in this index.
Chain of custody: Domestic warehouse despatch → submitter (2–8 °C, 10 d) → laboratory (courier, cold pack, 12 d) → assay.
Assay date: 05 Dec 2025
Submitted to the index: 23 Dec 2025
Laboratory: PeptideMeter, Tallinn, Estonia
Method basis: 28 min gradient · high-resolution MS
Verification status: VERIFIED

What this report does not establish

That the material is free of bacterial endotoxin. Endotoxin was not measured. No chromatographic result bears on it.
That the material is sterile. Sterility was not tested.
That any other vial in the same lot has the same composition. One vial was tested. Within-lot vial-to-vial variation in this index has a median of 0.28 pp and a 90th percentile of 0.74 pp.
That any other lot from the same supplier has the same composition. Batch-to-batch variation is larger than within-batch variation for every supplier here.
That the material is suitable for administration to a human or an animal. These are research compounds. No result on this site is a safety assessment and the index takes no position on use.
That the material was manufactured by the supplier that sold it. Contract synthesis is normal in this market and twenty-nine batches in the index fingerprint to a bulk from a different company than the one named on the label.

Section 6

Annotations 4

Signed technical comments from index contributors. Not a discussion forum — annotations question or contextualise the result, and the data team replies where a reply is warranted.

isotope_cluster23 Dec 2025

The isotope pattern is consistent with the elemental composition of GHRP-2. That is a second, independent check on identity and it agrees with the mass.

ms_confirm23 Dec 2025

Identity confirmed, purity 99.30 %, mass balance closed. This record is complete against the index definition of complete and there is nothing outstanding on it.

hplc_ola23 Dec 2025

Flow rate is the free variable most operators never touch. Halving it on an isocratic hold across the pair is a fifteen-minute experiment with a definitive answer.

osmolality_chk23 Dec 2025

Osmolality after reconstitution to a stated volume bounds total solids and gives an independent, crude check on the fill. Ten minutes and no peptide-specific reagents.

Section 7

Related records

Same batch — lot QYB-2510-D4215 n=10

Report	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2025-018533	QYB-2510-D4215	Nordanalyt	2025-Q4	99.02	4.06	—	Buyer-sourced	0	PASS
AX-2025-018534	QYB-2510-D4215	PeptideMeter	2025-Q4	99.04	4.68	105.7	Buyer-sourced	1	FAIL
AX-2025-018535	QYB-2510-D4215	Kestrel	2025-Q4	99.71	4.52	100.1	Buyer-sourced	0	PASS
AX-2025-018536	QYB-2510-D4215	Janoshik	2025-Q4	99.34	4.10	—	Buyer-sourced	0	PASS
AX-2025-018537	QYB-2510-D4215	Janoshik	2025-Q4	98.78	4.25	—	Buyer-sourced	0	FAIL
AX-2025-018538	QYB-2510-D4215	Janoshik	2025-Q4	98.96	4.36	104.0	Buyer-sourced	0	FAIL
AX-2025-018539	QYB-2510-D4215	Janoshik	2025-Q4	99.06	3.96	90.0	Buyer-sourced	1	FAIL
AX-2025-018540	QYB-2510-D4215	Janoshik	2025-Q4	98.87	4.30	103.5	Buyer-sourced	0	FAIL
AX-2025-018541	QYB-2510-D4215	VendorInvestigate	2025-Q4	99.24	4.68	—	Buyer-sourced	0	PASS

Batch rollup and reproducibility assessment →

Cross-sections

Other recent GHRP-2 records in the index.
Report	Supplier	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-043535	SIG	SIG-2603-6031	Janoshik	2026-Q3	99.12	1.26	105.7	Buyer-sourced	0	PASS
AX-2026-043541	SIG	SIG-2603-6031	PeptideMeter	2026-Q3	99.06	2.08	107.4	Buyer-sourced	1	MARGINAL
AX-2026-043539	SIG	SIG-2603-6031	Janoshik	2026-Q3	98.95	1.74	100.4	Buyer-sourced	1	FAIL
AX-2026-043534	SIG	SIG-2603-6031	Medutest	2026-Q3	99.11	2.15	89.8	Buyer-sourced	1	MARGINAL
AX-2026-043542	SIG	SIG-2603-6031	VendorInvestigate	2026-Q3	99.05	1.43	—	Buyer-sourced	1	FAIL
AX-2026-043543	SIG	SIG-2603-6031	Janoshik	2026-Q3	99.38	1.70	98.6	Buyer-sourced	0	PASS
AX-2026-043544	SIG	SIG-2603-6031	PeptideMeter	2026-Q3	99.57	1.51	105.4	Buyer-sourced	0	PASS
AX-2026-043538	SIG	SIG-2603-6031	Kestrel	2026-Q3	98.93	1.72	—	Buyer-sourced	0	FAIL

Other recent QYB records in the index.
Report	Compound	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-041471	PT-141 (Bremelanotide)	QYB-2607-L4875	Janoshik	2026-Q3	99.72	3.75	101.7	Buyer-sourced	0	PASS
AX-2026-041377	Ipamorelin	QYB-2605-H3558	Nordanalyt	2026-Q3	99.93	4.35	—	Buyer-sourced	0	PASS
AX-2026-041427	Retatrutide	QYB-2604-B4920	PeptideMeter	2026-Q3	97.93	2.45	101.7	Buyer-sourced	0	PASS
AX-2026-041428	Retatrutide	QYB-2604-B4920	Janoshik	2026-Q3	98.35	3.20	—	Laboratory-retained	0	PASS
AX-2026-041473	PT-141 (Bremelanotide)	QYB-2607-L4875	PeptideMeter	2026-Q3	99.58	3.74	94.4	Buyer-sourced	0	PASS
AX-2026-041472	PT-141 (Bremelanotide)	QYB-2607-L4875	PeptideMeter	2026-Q3	99.93	3.94	—	Buyer-sourced	0	PASS
AX-2026-041429	Retatrutide	QYB-2604-B4920	Medutest	2026-Q3	98.53	2.69	99.8	Buyer-sourced	1	MARGINAL
AX-2026-041431	Retatrutide	QYB-2604-B4920	Janoshik	2026-Q3	98.63	2.32	94.2	Buyer-sourced	0	PASS