AX-2025-020788 · CJC-1295 (no DAC) · CPC lot CPC-2509-L9867

Section 1

Results

Measured against the index acceptance specification for the growth-hormone axis peptides class. A specification is a decision the index made; a result is a measurement. See where each threshold came from.

Parameter	Method	Result	Unit	Index spec
Purity	RP-HPLC, 15 min gradient, 214 nm	97.79	% area	≥ 98.0	FAIL
Total impurities	RP-HPLC, sum of reported peaks	2.21	% area	≤ 2.0	FAIL
Largest single impurity	RP-HPLC — native GRF (1-29) (−59 Da)	1.08	% area	≤ 1.00	FAIL
Identity — theoretical monoisotopic mass	calculated from molecular formula	3365.7402	Da	—	NOT TESTED
Identity — observed monoisotopic mass	LC-MS, unit resolution (single quadrupole)	3365.7742	Da	—	NOT TESTED
Mass accuracy	observed vs theoretical	+10.1	ppm	± 15	PASS
Total peptide content	by difference from the gravimetric mass balance	88.95	%w/w	—	NOT TESTED
Target-compound content	total peptide content × purity	86.98	%w/w	—	NOT TESTED
Gross fill (as weighed)	five-place balance, under nitrogen	2.11	mg	2 mg declared	NOT TESTED
Recovered target peptide	gross fill × target content	1.835	mg	2 mg claim	NOT TESTED
Recovery against label claim	recovered ÷ claim	91.8	%	90.0 – 110.0	PASS
Water content	Karl Fischer, coulometric, duplicate	5.80	%	≤ 9.0	PASS
Counter-ion (acetate)	anion-exchange ion chromatography	4.44	%	≤ 12.0	PASS
Residual solvent + inorganic residue	estimated — see /methods/residual-solvents-q3c/	0.81	%	—	NOT TESTED
Endotoxin	kinetic chromogenic LAL	11.29	EU/mg	≤ 5.0	FAIL
Sterility	not ordered on this submission	not determined	—	no growth	NOT TESTED
Appearance	visual, diffuse light, black and white background	White cake, partial shrinkage from vial wall	—	white to off-white intact cake	FAIL
Reconstitution clarity	reconstituted per submitter procedure	Clear, colourless, no visible particulates	—	clear, no visible particulates	PASS
pH (reconstituted, 1 mg/mL)	potentiometric, two-point calibration	5.04	—	2.5 – 8.0	PASS
Retention time (observed)	RP-HPLC, 15 min gradient	6.86	min	6.94 ± 0.30 (normalised)	PASS
Peak asymmetry (10 % height)	Ph. Eur. 2.2.46	1.39	—	0.80 – 1.50	PASS
Plate count, main peak	N = 5.54 (t/w½)²	69,665	—	≥ 20,000	PASS
Resolution to nearest impurity	vs deamidated (+1 Da)	1.13	—	no index criterion	REPORTED

Section 2

Chromatogram and peak integration

The figure is drawn from the table. It is not an independent illustration — if a number in the table changed, the trace would change with it.

Figure 1. RP-HPLC trace, 15 min gradient, 214 nm. Drawn from the peak-integration table below: peak positions are the reported retention times, peak areas are the reported area percentages, peak widths follow the reported plate count of 69,665, and skew follows the reported asymmetry factor of 1.39. Baseline noise is seeded from the report ID and is illustrative. The lower panel is the same trace at a stated vertical expansion with the slow gradient drift subtracted, because at full scale a 1.08 % impurity against a 97.79 % main peak is a line two pixels tall; the main peak runs off-scale there by design.

#	Peak	RT	Area (µV·s)	% area	Δ mass (Da)
1	CJC-1295 (no DAC) — main peak	6.86	4,752,897	97.79	—
2	native GRF (1-29) (−59 Da)	6.69	52,491	1.08	-58.995
3	des-Tyr1 (−163 Da)	6.59	29,162	0.60	-163.063
4	deamidated (+1 Da)	6.74	13,609	0.28	+0.984
5	acetyl adduct (+42 Da)	7.08	6,804	0.14	+42.011
6	truncated (1-24)	6.28	5,346	0.11	-570.291
Closure check — purity + Σ impurities				100.00	CLOSES

Reporting threshold 0.05 % area. Peaks below it are not reported and are therefore excluded from the total, which overstates purity by their sum — see peak integration.

Section 3

Mass spectrum and identity

Theoretical monoisotopic mass 3365.7402 Da, computed from C152H252N44O42. All ppm figures on this site are against monoisotopic values — see why that matters.

Figure 2. ESI charge-state envelope, unit-resolution instrument. Sticks are placed at m/z = (M + z × 1.007276)/z for the observed neutral mass of 3365.7742 Da. Sodium (+21.982/z) and potassium (+37.956/z) adducts are shown where the parent charge state exceeds 25 % relative abundance; they are adducts of the same molecule and are not impurities.

Charge	Theoretical m/z	Observed m/z	Δ m/z	Rel. abundance (%)
5+	674.1553	674.1621	0.0068	8.0
4+	842.4423	842.4508	0.0085	53.3
3+	1122.9207	1122.9320	0.0113	100.0
2+	1683.8774	1683.8944	0.0170	56.4

Section 4

Gravimetric mass balance

Water, counter-ion, residual and total peptide content are exhaustive and mutually exclusive categories. They must account for 100 % of the cake mass. The index closes this to within 0.02 pp and the build asserts it.

Component	Determination	%w/w of cake
Water	Karl Fischer	5.80
Counter-ion (acetate)	ion chromatography	4.44
Residual solvent + inorganic residue	estimated	0.81
Total peptide content	by difference	88.95
Total		100.00 CLOSES

Supplier certificate, as declared

The supplier's own figures for this lot, beside the independent measurement. Divergence is assessed against the batch median, not against this single report — see rule AX-R05.

Figure	Supplier COA	This report	Index note
Purity (% area)	98.24	97.79	divergence +0.40 pp vs batch median
Total impurities (% area)	1.76	2.21
Largest single impurity (% area)	1.27	1.08
Water (%)	not stated	5.80
Counter-ion (%)	not stated	4.44
Peptide content (%w/w)	91.7	88.95
Endotoxin (EU/mg)	not stated	11.29
Method stated on certificate	NO	—	no method section — the result cannot be reconciled against any other

Section 5

Provenance and caveats

Where a sample came from changes the weight of the evidence. The index treats it as a first-class field.

Sample provenance: Buyer-sourced
Evidentiary weight: Strongest available evidence in this index.
Chain of custody: Retail purchase → submitter (2–8 °C, 21 d) → laboratory (courier, ambient, 3 d) → assay.
Assay date: 08 Nov 2025
Submitted to the index: 28 Nov 2025
Laboratory: Kestrel Bioanalytical, Melbourne, Australia
Method basis: 15 min gradient · unit-resolution MS
Verification status: IDENTITY CONSISTENT

What this report does not establish

That the compound is not a near-isobaric alternative. Identity was confirmed at unit mass resolution, which cannot exclude species differing by 1 or 2 Da. The specific alternatives not excluded are named in the identity block above.
That the material is sterile. Sterility was not tested.
That any other vial in the same lot has the same composition. One vial was tested. Within-lot vial-to-vial variation in this index has a median of 0.28 pp and a 90th percentile of 0.74 pp.
That any other lot from the same supplier has the same composition. Batch-to-batch variation is larger than within-batch variation for every supplier here.
That the material is suitable for administration to a human or an animal. These are research compounds. No result on this site is a safety assessment and the index takes no position on use.
That the material was manufactured by the supplier that sold it. Contract synthesis is normal in this market and twenty-nine batches in the index fingerprint to a bulk from a different company than the one named on the label.

Section 6

Annotations 4

Signed technical comments from index contributors. Not a discussion forum — annotations question or contextualise the result, and the data team replies where a reply is warranted.

resin_loading28 Nov 2025

High resin substitution on a difficult sequence produces on-resin aggregation, and on-resin aggregation produces exactly this impurity pattern. It is a fixable process parameter and one of the commonest causes of a result in this band.

peak_integrator28 Nov 2025

Resolution to the nearest named impurity is reported on this record with no verdict attached, which is the correct handling. A threshold on that figure would penalise compounds for their own impurity profiles.

gradient_2528 Nov 2025

Resolution scales with the square root of plate count and linearly with selectivity. Shallowing the gradient changes selectivity, which is the more effective of the two levers on a close pair.

ash_sulfated28 Nov 2025

Four slots — water, counter-ion, residual and peptide — are exhaustive and mutually exclusive. Anything that does not fit one of them is a fifth thing nobody measured, and on this record there is no remainder.

Section 7

Related records

Same batch — lot CPC-2509-L9867 n=4

Report	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2025-020785	CPC-2509-L9867	Janoshik	2025-Q4	97.86	6.46	99.6	Buyer-sourced	1	FAIL
AX-2025-020786	CPC-2509-L9867	Medutest	2025-Q4	97.99	5.96	—	Supplier-supplied	0	FAIL
AX-2025-020787	CPC-2509-L9867	Janoshik	2025-Q4	97.82	6.27	—	Buyer-sourced	0	FAIL

Batch rollup and reproducibility assessment →

Cross-sections

Other recent CJC-1295 (no DAC) records in the index.
Report	Supplier	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-042942	MKM	MKM-2603-V3346	Janoshik	2026-Q3	99.52	3.43	—	Supplier-supplied	0	PASS
AX-2026-041599	QSC	QSC-2606-V7416	VendorInvestigate	2026-Q3	97.96	3.52	—	Buyer-sourced	1	FAIL
AX-2026-042784	GGPeps	GGPE-2606-E1234	Medutest	2026-Q3	98.27	4.55	—	Buyer-sourced	0	PASS
AX-2026-042939	MKM	MKM-2603-V3346	PeptideMeter	2026-Q3	99.44	3.39	—	Buyer-sourced	0	PASS
AX-2026-041601	QSC	QSC-2606-V7416	Kestrel	2026-Q3	98.33	4.10	—	Buyer-sourced	1	MARGINAL
AX-2026-042945	MKM	MKM-2603-V3346	PeptideMeter	2026-Q3	99.39	3.54	—	Laboratory-retained	0	PASS
AX-2026-042786	GGPeps	GGPE-2606-E1234	Janoshik	2026-Q3	98.22	3.77	—	Buyer-sourced	0	PASS
AX-2026-041602	QSC	QSC-2606-V7416	VendorInvestigate	2026-Q3	98.36	3.38	101.8	Buyer-sourced	1	MARGINAL

Other recent CPC records in the index.
Report	Compound	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-043319	BPC-157	CPC-2602-W1610	Medutest	2026-Q3	98.82	2.88	103.5	Buyer-sourced	2	MARGINAL
AX-2026-043295	Liraglutide	CPC-2602-G9942	Janoshik	2026-Q3	97.34	5.17	106.3	Buyer-sourced	0	FAIL
AX-2026-043288	Tirzepatide	CPC-2607-L1516	Janoshik	2026-Q3	96.89	5.11	101.1	Laboratory-retained	0	FAIL
AX-2026-043327	N-Acetyl Semax Amide	CPC-2602-D1905	Nordanalyt	2026-Q3	99.05	5.67	90.6	Buyer-sourced	1	FAIL
AX-2026-043328	N-Acetyl Semax Amide	CPC-2602-D1905	Janoshik	2026-Q3	99.49	6.83	100.9	Supplier-supplied	0	PASS
AX-2026-043257	L-Carnosine	CPC-2603-G9615	VendorInvestigate	2026-Q3	98.20	10.41	102.2	Buyer-sourced	0	FAIL
AX-2026-043297	Liraglutide	CPC-2602-G9942	Janoshik	2026-Q3	97.01	4.95	103.5	Buyer-sourced	0	FAIL
AX-2026-043331	N-Acetyl Semax Amide	CPC-2602-D1905	Janoshik	2026-Q3	99.46	6.22	—	Supplier-supplied	0	PASS