AX-2024-005198 · GHRP-6 · CPC lot CPC-2401-N1934

Section 1

Results

Measured against the index acceptance specification for the growth-hormone axis peptides class. A specification is a decision the index made; a result is a measurement. See where each threshold came from.

Parameter	Method	Result	Unit	Index spec
Purity	RP-HPLC, 32 min gradient, 214 nm	99.58	% area	≥ 99.0	PASS
Total impurities	RP-HPLC, sum of reported peaks	0.42	% area	≤ 1.0	PASS
Largest single impurity	RP-HPLC — [D-Trp]-epimer	0.29	% area	≤ 1.00	PASS
Identity — theoretical monoisotopic mass	calculated from molecular formula	872.4446	Da	—	NOT TESTED
Identity — observed monoisotopic mass	LC-MS, high resolution (ESI-TOF/QTOF)	872.4428	Da	—	NOT TESTED
Mass accuracy	observed vs theoretical	-2.1	ppm	± 15	PASS
Total peptide content	by difference from the gravimetric mass balance	87.14	%w/w	—	NOT TESTED
Target-compound content	total peptide content × purity	86.77	%w/w	—	NOT TESTED
Gross fill (as weighed)	five-place balance, under nitrogen	5.80	mg	5 mg declared	NOT TESTED
Recovered target peptide	gross fill × target content	5.033	mg	5 mg claim	NOT TESTED
Recovery against label claim	recovered ÷ claim	100.7	%	90.0 – 110.0	PASS
Water content	Karl Fischer, coulometric, duplicate	5.48	%	≤ 9.0	PASS
Counter-ion (acetate)	anion-exchange ion chromatography	6.93	%	≤ 12.0	PASS
Residual solvent + inorganic residue	estimated — see /methods/residual-solvents-q3c/	0.45	%	—	NOT TESTED
Endotoxin	kinetic chromogenic LAL	0.52	EU/mg	≤ 5.0	PASS
Sterility	not ordered on this submission	not determined	—	no growth	NOT TESTED
Appearance	visual, diffuse light, black and white background	White lyophilised cake, intact	—	white to off-white intact cake	PASS
Reconstitution clarity	reconstituted per submitter procedure	Clear, colourless, no visible particulates	—	clear, no visible particulates	PASS
pH (reconstituted, 1 mg/mL)	potentiometric, two-point calibration	5.28	—	2.5 – 8.0	PASS
Retention time (observed)	RP-HPLC, 32 min gradient	8.94	min	8.96 ± 0.25 (normalised)	PASS
Peak asymmetry (10 % height)	Ph. Eur. 2.2.46	0.88	—	0.80 – 1.50	PASS
Plate count, main peak	N = 5.54 (t/w½)²	76,063	—	≥ 20,000	PASS
Resolution to nearest impurity	vs [D-Trp]-epimer	0.96	—	no index criterion	REPORTED

Section 2

Chromatogram and peak integration

The figure is drawn from the table. It is not an independent illustration — if a number in the table changed, the trace would change with it.

Figure 1. RP-HPLC trace, 32 min gradient, 214 nm. Drawn from the peak-integration table below: peak positions are the reported retention times, peak areas are the reported area percentages, peak widths follow the reported plate count of 76,063, and skew follows the reported asymmetry factor of 0.88. Baseline noise is seeded from the report ID and is illustrative. The lower panel is the same trace at a stated vertical expansion with the slow gradient drift subtracted, because at full scale a 0.29 % impurity against a 99.58 % main peak is a line two pixels tall; the main peak runs off-scale there by design.

#	Peak	RT	Area (µV·s)	% area	Δ mass (Da)
1	GHRP-6 — main peak	8.94	4,839,897	99.58	—
2	[D-Trp]-epimer	8.81	14,095	0.29	0.000
3	oxidised Trp (+16 Da)	8.58	3,888	0.08	+15.995
4	des-Lys (−128 Da)	8.71	1,458	0.03	-128.095
5	bis-oxidised Trp (+32 Da)	8.32	972	0.02	+31.990
Closure check — purity + Σ impurities				100.00	CLOSES

Reporting threshold 0.05 % area. Peaks below it are not reported and are therefore excluded from the total, which overstates purity by their sum — see peak integration.

Section 3

Mass spectrum and identity

Theoretical monoisotopic mass 872.4446 Da, computed from C46H56N12O6. All ppm figures on this site are against monoisotopic values — see why that matters.

Figure 2. ESI charge-state envelope, high-resolution instrument. Sticks are placed at m/z = (M + z × 1.007276)/z for the observed neutral mass of 872.4428 Da. Sodium (+21.982/z) and potassium (+37.956/z) adducts are shown where the parent charge state exceeds 25 % relative abundance; they are adducts of the same molecule and are not impurities.

Charge	Theoretical m/z	Observed m/z	Δ m/z	Rel. abundance (%)
2+	437.2296	437.2287	-0.0009	50.6
1+	873.4519	873.4501	-0.0018	100.0

Section 4

Gravimetric mass balance

Water, counter-ion, residual and total peptide content are exhaustive and mutually exclusive categories. They must account for 100 % of the cake mass. The index closes this to within 0.02 pp and the build asserts it.

Component	Determination	%w/w of cake
Water	Karl Fischer	5.48
Counter-ion (acetate)	ion chromatography	6.93
Residual solvent + inorganic residue	estimated	0.45
Total peptide content	by difference	87.14
Total		100.00 CLOSES

Supplier certificate, as declared

The supplier's own figures for this lot, beside the independent measurement. Divergence is assessed against the batch median, not against this single report — see rule AX-R05.

Figure	Supplier COA	This report	Index note
Purity (% area)	99.41	99.58	divergence -0.21 pp vs batch median
Total impurities (% area)	0.32	0.42
Largest single impurity (% area)	0.14	0.29
Water (%)	4.9	5.48
Counter-ion (%)	3.5	6.93
Peptide content (%w/w)	91.0	87.14
Endotoxin (EU/mg)	not stated	0.52
Method stated on certificate	YES	—	column, gradient and wavelength given

Section 5

Provenance and caveats

Where a sample came from changes the weight of the evidence. The index treats it as a first-class field.

Sample provenance: Buyer-sourced
Evidentiary weight: Strongest available evidence in this index.
Chain of custody: Retail purchase → third-party forwarder (14 d) → laboratory (courier, cold pack, 6 d) → assay.
Assay date: 26 Jun 2024
Submitted to the index: 17 Jul 2024
Laboratory: VendorInvestigate, Riga, Latvia
Method basis: 32 min gradient · high-resolution MS
Verification status: VERIFIED

What this report does not establish

That the material is sterile. Sterility was not tested.
That any other vial in the same lot has the same composition. One vial was tested. Within-lot vial-to-vial variation in this index has a median of 0.28 pp and a 90th percentile of 0.74 pp.
That any other lot from the same supplier has the same composition. Batch-to-batch variation is larger than within-batch variation for every supplier here.
That the material is suitable for administration to a human or an animal. These are research compounds. No result on this site is a safety assessment and the index takes no position on use.
That the material was manufactured by the supplier that sold it. Contract synthesis is normal in this market and twenty-nine batches in the index fingerprint to a bulk from a different company than the one named on the label.

Section 6

Annotations 4

Signed technical comments from index contributors. Not a discussion forum — annotations question or contextualise the result, and the data team replies where a reply is warranted.

baseline_noise17 Jul 2024

Baseline on this trace is flat enough that the reporting threshold is doing real work rather than hiding peaks in noise. That is a precondition for believing 99.58 % and it is not always met.

retest_date17 Jul 2024

A retest date on the certificate would tell you what the supplier is actually asserting about stability. Purity at 99.58 % on assay day is a starting condition, not a shelf life.

ms_confirm17 Jul 2024

A reference standard on the same sequence would upgrade this from a good mass to a traceable one. The distinction matters where a result is going to be cited rather than merely read.

deamidation_rate17 Jul 2024

Deamidation adds one dalton, which at this mass is close to the resolution of the question rather than to the resolution of the instrument. The named-impurity table is the better place to look for it.

Section 7

Related records

Same batch — lot CPC-2401-N1934 n=10

Report	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2024-005195	CPC-2401-N1934	Janoshik	2024-Q2	99.93	5.46	99.9	Buyer-sourced	1	MARGINAL
AX-2024-005196	CPC-2401-N1934	Janoshik	2024-Q2	99.72	5.13	102.2	Buyer-sourced	1	MARGINAL
AX-2024-005197	CPC-2401-N1934	VendorInvestigate	2024-Q2	99.49	5.51	—	Buyer-sourced	1	MARGINAL
AX-2024-005199	CPC-2401-N1934	Janoshik	2024-Q2	99.84	4.89	—	Buyer-sourced	1	MARGINAL
AX-2024-005200	CPC-2401-N1934	PeptideMeter	2024-Q2	99.54	5.02	103.6	Buyer-sourced	1	MARGINAL
AX-2024-005201	CPC-2401-N1934	PeptideMeter	2024-Q2	99.65	5.67	—	Buyer-sourced	1	MARGINAL
AX-2024-005202	CPC-2401-N1934	Janoshik	2024-Q2	99.58	4.90	—	Buyer-sourced	1	MARGINAL
AX-2024-005203	CPC-2401-N1934	VendorInvestigate	2024-Q2	99.60	5.47	—	Buyer-sourced	2	MARGINAL
AX-2024-005204	CPC-2401-N1934	Janoshik	2024-Q2	99.64	5.47	—	Buyer-sourced	1	MARGINAL

Batch rollup and reproducibility assessment →

Cross-sections

Other recent GHRP-6 records in the index.
Report	Supplier	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-042012	AMB	AMB-2603-S5667	Janoshik	2026-Q3	99.37	5.90	88.8	Laboratory-retained	1	MARGINAL
AX-2026-042528	HJ	HJ-2603-R5329	VendorInvestigate	2026-Q3	98.69	1.87	95.8	Buyer-sourced	0	FAIL
AX-2026-042015	AMB	AMB-2603-S5667	Janoshik	2026-Q3	99.54	6.44	97.1	Buyer-sourced	0	PASS
AX-2026-042526	HJ	HJ-2603-R5329	Medutest	2026-Q3	99.37	3.06	—	Laboratory-retained	0	PASS
AX-2026-042527	HJ	HJ-2603-R5329	VendorInvestigate	2026-Q3	99.21	2.77	93.2	Buyer-sourced	0	PASS
AX-2026-042525	HJ	HJ-2603-R5329	Janoshik	2026-Q3	99.52	3.24	92.0	Buyer-sourced	0	PASS
AX-2026-042013	AMB	AMB-2603-S5667	Kestrel	2026-Q3	99.93	5.75	—	Buyer-sourced	0	PASS
AX-2026-042017	AMB	AMB-2603-S5667	Medutest	2026-Q3	99.76	6.42	97.4	Buyer-sourced	0	PASS

Other recent CPC records in the index.
Report	Compound	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-043319	BPC-157	CPC-2602-W1610	Medutest	2026-Q3	98.82	2.88	103.5	Buyer-sourced	2	MARGINAL
AX-2026-043295	Liraglutide	CPC-2602-G9942	Janoshik	2026-Q3	97.34	5.17	106.3	Buyer-sourced	0	FAIL
AX-2026-043288	Tirzepatide	CPC-2607-L1516	Janoshik	2026-Q3	96.89	5.11	101.1	Laboratory-retained	0	FAIL
AX-2026-043327	N-Acetyl Semax Amide	CPC-2602-D1905	Nordanalyt	2026-Q3	99.05	5.67	90.6	Buyer-sourced	1	FAIL
AX-2026-043328	N-Acetyl Semax Amide	CPC-2602-D1905	Janoshik	2026-Q3	99.49	6.83	100.9	Supplier-supplied	0	PASS
AX-2026-043257	L-Carnosine	CPC-2603-G9615	VendorInvestigate	2026-Q3	98.20	10.41	102.2	Buyer-sourced	0	FAIL
AX-2026-043297	Liraglutide	CPC-2602-G9942	Janoshik	2026-Q3	97.01	4.95	103.5	Buyer-sourced	0	FAIL
AX-2026-043331	N-Acetyl Semax Amide	CPC-2602-D1905	Janoshik	2026-Q3	99.46	6.22	—	Supplier-supplied	0	PASS