AX-2025-020367 · 5-Amino-1MQ · MKM lot MKM-2503-H3929

Section 1

Results

Measured against the index acceptance specification for the non-peptide adjuncts class. A specification is a decision the index made; a result is a measurement. See where each threshold came from.

Parameter	Method	Result	Unit	Index spec
Purity	RP-HPLC, 30 min gradient, 214 nm	99.70	% area	≥ 98.0	PASS
Total impurities	RP-HPLC, sum of reported peaks	0.30	% area	≤ 2.0	PASS
Largest single impurity	RP-HPLC — 1-methylquinolinium (des-amino, −15 Da)	0.12	% area	≤ 1.00	PASS
Identity — theoretical monoisotopic mass	calculated from molecular formula	285.9967	Da	—	NOT TESTED
Identity — observed monoisotopic mass	LC-MS, high resolution (ESI-TOF/QTOF)	285.9962	Da	—	NOT TESTED
Mass accuracy	observed vs theoretical	-1.9	ppm	± 10	PASS
Total peptide content	by difference from the gravimetric mass balance	53.98	%w/w	—	NOT TESTED
Target-compound content	total peptide content × purity	53.82	%w/w	—	NOT TESTED
Gross fill (as weighed)	five-place balance, under nitrogen	198.63	mg	100 mg declared	NOT TESTED
Recovered target peptide	gross fill × target content	106.903	mg	100 mg claim	NOT TESTED
Recovery against label claim	recovered ÷ claim	106.9	%	90.0 – 110.0	PASS
Water content	Karl Fischer, coulometric, duplicate	5.09	%	≤ 10.0	PASS
Counter-ion (iodide)	anion-exchange ion chromatography	40.15	%	≤ 12.0	FAIL
Residual solvent + inorganic residue	estimated — see /methods/residual-solvents-q3c/	0.78	%	—	NOT TESTED
Endotoxin	kinetic chromogenic LAL	0.67	EU/mg	≤ 5.0	PASS
Sterility	not ordered on this submission	not determined	—	no growth	NOT TESTED
Appearance	visual, diffuse light, black and white background	Off-white lyophilised cake, intact	—	white to off-white intact cake	PASS
Reconstitution clarity	reconstituted per submitter procedure	Clear, colourless, no visible particulates	—	clear, no visible particulates	PASS
pH (reconstituted, 1 mg/mL)	potentiometric, two-point calibration	5.32	—	2.5 – 8.0	PASS
Retention time (observed)	RP-HPLC, 30 min gradient	5.50	min	5.42 ± 0.25 (normalised)	PASS
Peak asymmetry (10 % height)	Ph. Eur. 2.2.46	1.01	—	0.80 – 1.50	PASS
Plate count, main peak	N = 5.54 (t/w½)²	70,981	—	≥ 20,000	PASS
Resolution to nearest impurity	vs free iodide (counter-ion, by IC)	0.00	—	no index criterion	REPORTED

Section 2

Chromatogram and peak integration

The figure is drawn from the table. It is not an independent illustration — if a number in the table changed, the trace would change with it.

Figure 1. RP-HPLC trace, 30 min gradient, 214 nm. Drawn from the peak-integration table below: peak positions are the reported retention times, peak areas are the reported area percentages, peak widths follow the reported plate count of 70,981, and skew follows the reported asymmetry factor of 1.01. Baseline noise is seeded from the report ID and is illustrative. The lower panel is the same trace at a stated vertical expansion with the slow gradient drift subtracted, because at full scale a 0.12 % impurity against a 99.70 % main peak is a line two pixels tall; the main peak runs off-scale there by design.

#	Peak	RT	Area (µV·s)	% area	Δ mass (Da)
1	5-Amino-1MQ — main peak	5.50	4,845,729	99.70	—
2	1-methylquinolinium (des-amino, −15 Da)	5.18	5,832	0.12	-15.011
3	free iodide (counter-ion, by IC)	5.50	5,832	0.12	0.000
4	5-nitro-1MQ (unreduced precursor, +30 Da)	6.18	1,458	0.03	+29.974
5	8-amino regioisomer	5.72	1,458	0.03	0.000
Closure check — purity + Σ impurities				100.00	CLOSES

Reporting threshold 0.05 % area. Peaks below it are not reported and are therefore excluded from the total, which overstates purity by their sum — see peak integration.

Section 3

Mass spectrum and identity

Theoretical monoisotopic mass 285.9967 Da, computed from C10H11N2+ · I−. All ppm figures on this site are against monoisotopic values — see why that matters.

Figure 2. ESI charge-state envelope, high-resolution instrument. Sticks are placed at m/z = (M + z × 1.007276)/z for the observed neutral mass of 285.9962 Da. Sodium (+21.982/z) and potassium (+37.956/z) adducts are shown where the parent charge state exceeds 25 % relative abundance; they are adducts of the same molecule and are not impurities.

Charge	Theoretical m/z	Observed m/z	Δ m/z	Rel. abundance (%)
1+	287.0040	287.0035	-0.0005	100.0

Section 4

Gravimetric mass balance

Water, counter-ion, residual and total peptide content are exhaustive and mutually exclusive categories. They must account for 100 % of the cake mass. The index closes this to within 0.02 pp and the build asserts it.

Component	Determination	%w/w of cake
Water	Karl Fischer	5.09
Counter-ion (iodide)	ion chromatography	40.15
Residual solvent + inorganic residue	estimated	0.78
Total peptide content	by difference	53.98
Total		100.00 CLOSES

Supplier certificate, as declared

The supplier's own figures for this lot, beside the independent measurement. Divergence is assessed against the batch median, not against this single report — see rule AX-R05.

Figure	Supplier COA	This report	Index note
Purity (% area)	99.83	99.70	divergence +0.25 pp vs batch median
Total impurities (% area)	not stated	0.30
Largest single impurity (% area)	not stated	0.12
Water (%)	4.2	5.09
Counter-ion (%)	not stated	40.15
Peptide content (%w/w)	not stated	53.98
Endotoxin (EU/mg)	0.64	0.67
Method stated on certificate	YES	—	column, gradient and wavelength given

Section 5

Provenance and caveats

Where a sample came from changes the weight of the evidence. The index treats it as a first-class field.

Sample provenance: Buyer-sourced
Evidentiary weight: Strongest available evidence in this index.
Chain of custody: Retail purchase → submitter (2–8 °C, 3 d) → laboratory (courier, ambient, 4 d) → assay.
Assay date: 09 Sep 2025
Submitted to the index: 18 Sep 2025
Laboratory: Janoshik Analytical, Ostrava, Czechia
Method basis: 30 min gradient · high-resolution MS
Verification status: VERIFIED

What this report does not establish

That the material is sterile. Sterility was not tested.
That any other vial in the same lot has the same composition. One vial was tested. Within-lot vial-to-vial variation in this index has a median of 0.28 pp and a 90th percentile of 0.74 pp.
That any other lot from the same supplier has the same composition. Batch-to-batch variation is larger than within-batch variation for every supplier here.
That the material is suitable for administration to a human or an animal. These are research compounds. No result on this site is a safety assessment and the index takes no position on use.
That the material was manufactured by the supplier that sold it. Contract synthesis is normal in this market and twenty-nine batches in the index fingerprint to a bulk from a different company than the one named on the label.

Section 6

Annotations 3

Signed technical comments from index contributors. Not a discussion forum — annotations question or contextualise the result, and the data team replies where a reply is warranted.

quad_unit18 Sep 2025

Instrument class is on the record, which is what allows -1.9 ppm to be read at its proper weight. The index distinguishes "confirmed" from "consistent with" precisely on this field.

schema_v218 Sep 2025

Both the theoretical and the observed monoisotopic mass are in the published dataset, so the ppm figure can be re-derived rather than trusted. That is the intent of publishing both.

buyer_sourced18 Sep 2025

Ordering under an unremarkable name and address is the whole method. It requires no cooperation from anybody and it produces the strongest evidence this index holds.

Section 7

Related records

Same batch — lot MKM-2503-H3929 n=10

Report	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2025-020358	MKM-2503-H3929	Medutest	2025-Q3	99.57	5.36	104.4	Buyer-sourced	1	FAIL
AX-2025-020359	MKM-2503-H3929	Janoshik	2025-Q3	99.40	5.12	—	Buyer-sourced	0	FAIL
AX-2025-020360	MKM-2503-H3929	Nordanalyt	2025-Q3	99.46	5.57	—	Buyer-sourced	0	FAIL
AX-2025-020361	MKM-2503-H3929	Medutest	2025-Q3	99.59	4.92	94.4	Buyer-sourced	0	FAIL
AX-2025-020362	MKM-2503-H3929	Janoshik	2025-Q3	99.68	4.85	—	Buyer-sourced	0	FAIL
AX-2025-020363	MKM-2503-H3929	Janoshik	2025-Q3	99.68	5.52	95.1	Buyer-sourced	0	FAIL
AX-2025-020364	MKM-2503-H3929	PeptideMeter	2025-Q3	99.52	5.49	—	Buyer-sourced	0	FAIL
AX-2025-020365	MKM-2503-H3929	Kestrel	2025-Q3	99.86	5.37	97.3	Buyer-sourced	0	FAIL
AX-2025-020366	MKM-2503-H3929	Janoshik	2025-Q3	99.01	5.45	104.3	Buyer-sourced	0	FAIL

Batch rollup and reproducibility assessment →

Cross-sections

Other recent 5-Amino-1MQ records in the index.
Report	Supplier	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-043050	KP	KP-2511-W5347	Janoshik	2026-Q2	99.35	4.16	105.5	Buyer-sourced	0	FAIL
AX-2026-043044	KP	KP-2511-W5347	PeptideMeter	2026-Q2	99.61	4.15	90.9	Buyer-sourced	0	FAIL
AX-2026-043052	KP	KP-2511-W5347	Medutest	2026-Q2	99.45	4.23	105.5	Buyer-sourced	1	FAIL
AX-2026-043045	KP	KP-2511-W5347	Janoshik	2026-Q2	99.47	4.08	—	Buyer-sourced	0	FAIL
AX-2026-043043	KP	KP-2511-W5347	Janoshik	2026-Q2	99.58	4.16	101.1	Buyer-sourced	0	FAIL
AX-2026-042869	GGPeps	GGPE-2512-M2434	Kestrel	2026-Q2	99.55	4.65	103.9	Buyer-sourced	0	FAIL
AX-2026-042868	GGPeps	GGPE-2512-M2434	PeptideMeter	2026-Q2	99.32	4.39	88.5	Buyer-sourced	1	FAIL
AX-2026-043047	KP	KP-2511-W5347	Janoshik	2026-Q2	99.68	4.17	104.0	Supplier-supplied	0	FAIL

Other recent MKM records in the index.
Report	Compound	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Result
AX-2026-042942	CJC-1295 (no DAC)	MKM-2603-V3346	Janoshik	2026-Q3	99.52	3.43	—	Supplier-supplied	PASS
AX-2026-043019	Adipotide	MKM-2606-K5012	Janoshik	2026-Q3	97.61	5.77	105.1	Buyer-sourced	PASS
AX-2026-042939	CJC-1295 (no DAC)	MKM-2603-V3346	PeptideMeter	2026-Q3	99.44	3.39	—	Buyer-sourced	PASS
AX-2026-043020	Adipotide	MKM-2606-K5012	Medutest	2026-Q3	97.73	5.74	—	Buyer-sourced	PASS
AX-2026-043022	Adipotide	MKM-2606-K5012	Kestrel	2026-Q3	97.69	6.33	106.9	Buyer-sourced	PASS
AX-2026-043023	Adipotide	MKM-2606-K5012	Medutest	2026-Q3	97.48	5.56	103.7	Supplier-supplied	PASS
AX-2026-042945	CJC-1295 (no DAC)	MKM-2603-V3346	PeptideMeter	2026-Q3	99.39	3.54	—	Laboratory-retained	PASS
AX-2026-042938	CJC-1295 (no DAC)	MKM-2603-V3346	Nordanalyt	2026-Q3	99.47	3.67	95.0	Buyer-sourced	PASS